食品科学 ›› 2009, Vol. 30 ›› Issue (17 ): 278-281.doi: 10.7506/spkx1002-6630-200917064

• 生物工程 • 上一篇    下一篇

抗氧化剂对运动发酵单胞菌有氧胁迫和生长代谢的影响

陈 玲1,2,王成涛2 ,*,童军茂1,孙宝国2,曹雁平2   

  1. 1.石河子大学食品学院 2.北京工商大学化学与环境工程学院
  • 收稿日期:2009-05-22 出版日期:2009-09-01 发布日期:2014-04-14
  • 通讯作者: 王成涛2 ,*, E-mail:wct5566@163.com
  • 基金资助:

    国家“973”重点基础研究发展计划项目(2007CB707802)

Effects of Antioxidants on Growth and Metabolism in Zymomonas mobilis Under Oxidative Stress

CHEN Ling1,2,WANG Cheng-tao2,*,TONG Jun-mao1,SUN Bao-guo2,CAO Yan-ping2   

  1. 1. College of Food, Shihezi University, Shihezi 832003, China;
    2.College of Chemistry and Environment Engineering, Beijing Technology and Business University, Beijing 100048,China
  • Received:2009-05-22 Online:2009-09-01 Published:2014-04-14
  • Contact: WANG Cheng-tao2,*, E-mail:wct5566@163.com

摘要:

以运动发酵单胞菌(Z.mobilis)ATCC31821 为模式菌株,在发酵培养基中添加不同种类和剂量抗氧化剂,研究抗氧化剂对Z.mobilis抗氧胁迫性和生长代谢的影响。结果发现添加0.1~0.5mg/ml儿茶酚最有益于运动发酵单胞菌的菌体生长和乙醇发酵,有助于提高菌株的抗氧胁迫能力。通过正交试验对该菌的pH 值、装液量、摇床频率、儿茶酚浓度进行优化。结果表明:pH5,装液量200ml,摇床频率100r/min,儿茶酚0.1mg/ml,可达最大乙醇产量约为40.71g/L,较未添加儿茶酚抗氧化剂,其乙醇得率提高了5.33%。

关键词: 运动发酵单胞菌, 氧胁迫, 抗氧化剂, 发酵, 乙醇

Abstract:

In order to study the effects of antioxidants on growth and metabolism under oxidative stress, different types and doses of antioxidants were added in the fermentation medium of Zymomonas mobilis strain ATCC31821. It was found that pyrocatechol was more useful for growth and ethanol fermentation of Z. mobilis, and improving anti-oxidation stress ability. The optimized conditions obtained by orthogonal test were as follows, pH 5, 200 ml liquid volume, 100 r/min shaker frequency and 0.1 mg/ml pyrocatechol concentration. The maximum ethanol yield could reach 40.16 g/L under the optimized condition.

Key words: Zymomonas mobilis, oxidative stress, antioxidant, fermentation, ethanol

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