食品科学 ›› 2008, Vol. 29 ›› Issue (7): 133-137.

• 工艺技术 • 上一篇    下一篇

高压脉冲电场和超声波协同作用破碎啤酒废酵母的研究

谢阁,杨瑞金,卢蓉蓉,张文斌,赵伟   

  1. 江南大学食品科学与技术国家重点实验室; 江南大学食品学院; 江南大学食品科学与技术国家重点实验室 江苏无锡214122; 江苏无锡214122
  • 出版日期:2008-07-15 发布日期:2011-07-28

Breaking of Waste Brewer Yeast with Pulsed Electric Fields Combined with Ultrasound Treatment

 XIE  Ge, YANG  Rui-Jin, LU  Rong-Rong, ZHANG  Wen-Bin, ZHAO  Wei   

  1. 1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 2. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
  • Online:2008-07-15 Published:2011-07-28

摘要: 利用高压脉冲电场(PEF)与超声波技术相结合的方法破壁啤酒酵母细胞提取其中蛋白质和核酸。结果表明:啤酒酵母悬浮液先经高压脉冲处理(场强25kV/cm,脉冲频率667pps,处理时间1584μs)再经超声处理(功率400W,超声处理时间15min)后进行蛋白质和核酸提取,蛋白质和核酸提取率分别达到45.86%和53.75%,且蛋白质和核酸提取率均是PEF单独作用时的1.5倍左右,是超声波单独作用时的2倍左右。

关键词: 啤酒废酵母, 高压脉冲电场, 超声波, 蛋白质, 核酸

Abstract: Combined treatment with pulsed electric fields (PEF) and ultrasound was used to extract protein and nucleic acid from waste brewer yeast cell. Experiment was carried out with PEF (667 pps at 25 kV/cm, 1584μs) treatment followed by ultrasound (400 W, 15 min) treatment. The results showed that the highest extraction rate of protein and nucleic acid reaches 45.86% and 53.75%, respectively, almost 1.5 times of extraction rate of protein and nucleic acid only by PEF, and 2 times only by ultrasound.

Key words: waste brewer yeast, pulsed electric fields (PEF), ultrasound, protein, nucleic acid