食品科学 ›› 2008, Vol. 29 ›› Issue (7): 217-220.

• 工艺技术 • 上一篇    下一篇

山药糖蛋白的分离纯化与鉴定

丁青芝,马海乐,骆琳,李金忠   

  1. 江苏大学食品与生物工程学院; 江苏大学食品与生物工程学院 江苏镇江212013; 江苏镇江212013江苏省农产品生物加工与分离工程技术研究中心; 江苏镇江212013
  • 出版日期:2008-07-15 发布日期:2011-07-28

Study on Isolation, Purification and Identification of Glycoprtein from Chinese Yam

 DING  Qing-Zhi, MA  Hai-Le, LUO  Lin, LI  Jin-Zhong   

  1. 1.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; 2.Jiangsu Province Research Center of Bio-process and Separation of Agri-products, Zhenjiang 212013, China
  • Online:2008-07-15 Published:2011-07-28

摘要: 山药粗糖蛋白用DEAE-52纤维素层析纯化,得到了一种中性糖蛋白组分(GLP),用Sephadex G-75进一步进行层析;用薄层层析鉴定了GLP的纯度。用Sephadex G-75柱层析测定了GLP的分子量为89381D;薄层层析法测定了GLP的单糖组成,结果表明,GLP的单糖组成主要有:L-半乳糖、D-葡萄糖、D-甘露糖;对GLP进行了红外光谱扫描,其特征吸收峰再次表明该组分为一种糖蛋白。

关键词: 山药, 糖蛋白, 纯化, 性质

Abstract: Glycoprtein from Chinese yam purified by DEAE-cellulose 52. It was successful to obtain one fraction GLP by the step gradient elution. GLP was further purified by Sephadex G-75. TLC was applied to identify the purity of the GLP. The molecular weight of GLP measured by Sephadex G-75 is 89381 D. The experimental result of TLC revealed that the monosac-charide components of GLP are L-galactose, D-glucose and D-mannose. GLP is confirmed by its characteristic absorption peak of infrared spectrum.

Key words: Chinese yam, glycoprtein, purification, character