食品科学 ›› 2008, Vol. 29 ›› Issue (7): 221-225.

• 工艺技术 • 上一篇    下一篇

超声波辅助纤维素酶-碱液提取大枣皮红色素工艺的响应面优化研究

韩立宏,马涛   

  1. 宁夏师范学院化学与化学工程学院; 沈阳农业大学食品学院 宁夏固原756000; 辽宁沈阳110161
  • 出版日期:2008-07-15 发布日期:2011-07-28

Study on Optimization of Extraction of Red Pigment from Jujube Peel with Ultrasonic-assisted Cellulase-Alkaline Solution by Response Surface Analysis

 HAN  Li-Hong, MA  Tao   

  1. 1.Department of Chemistry and Chemical Technology, Ningxia Normal College, Guyuan 756000, China; 2. College of Food Science, Shenyang Agricultural University, Shenyang 110161, China
  • Online:2008-07-15 Published:2011-07-28

摘要: 本实验研究了纤维素酶解提取大枣皮红色素的最佳工艺。运用响应面分析法筛选出了纤维素酶的最佳酶解条件;通过正交试验设计确定了最佳碱液提取条件。结果表明:当纤维素酶用量为底物的1.26%、酶解温度51.90℃、酶解体系pH值5.19、酶解时间60min、碱液浓度1.0%、提取温度80℃、提取时间35min时,大枣皮红色素的提取率最高,达到1.326μg/g。

关键词: 纤维素酶, 大枣, 红色素, 碱液, 响应面分析

Abstract: In this study, the optimal conditions of cellulase reaction for destroying cell wall of jujube peel were determined by response surface analysis (RSA), and extraction conditions of pigment with alkaline solution were optimized by orthogonal test. The optimal parameters of cellulase reaction are quality of cellulase 1.26%, enzyme reaction temperature 51.90 ℃, pH value 5.19, and enzyme reaction time 60 min. And the optimal parameters of extraction with alkaline solution are alkaline solution concentration 1.0%, extraction temperature 80 ℃, extraction time 35 min. Under these conditions, the extraction rate of red pigment is up to 1.326μg/g.

Key words: cellulase, jujube, red pigment, alkaline solution, response surface analysis