食品科学 ›› 2008, Vol. 29 ›› Issue (7): 247-250.

• 生物工程 • 上一篇    下一篇

主成分分析法在花生四烯酸高产菌株代谢组学研究中的应用

李若昕,金明杰,彭超,潘晨亮,李霜,黄和   

  1. 华东理工大学生物反应器工程国家重点实验室; 南京工业大学制药与生命科学学院; 南京工业大学制药与生命科学学院 上海200237南京工业大学制药与生命科学学院; 江苏南京210009
  • 出版日期:2008-07-15 发布日期:2011-07-28

Application of Principal Component Analysis Method in Metabolomics Analysis of High Arachidonic Acid-producing Stains

LI  Ruo-Xin, JIN  Ming-Jie, PENG  Chao, PAN  Chen-Liang, LI  Shuang, HUANG  He   

  1. 1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; 2.College of Pharmacy and Life Science, Nanjing University of Technology, Nanjing 210009, China
  • Online:2008-07-15 Published:2011-07-28

摘要: 利用主成分分析法对于三株花生四烯酸高产高山被孢霉菌株的GC-MS谱图信息进行了分析,以菌株间通含的18种代谢组分为原始指标,提取出两个主成分变量,在二元主成分平面图上实现了对三个具有不同性状菌株的良好区分,同时发现主成分1可能为指向花生四烯酸积累的主成分指标,主成分2可能为指向菌体生长的主成分指标。

关键词: 主成分分析, 花生四烯酸, 高山被孢霉, 代谢组学, GC-MS检测

Abstract: The information on GC-MS spectra of three strains of high arachidonic acid-producing Mortierella alpine was analysed by principal component analysis, using 18 common metabolites as the original variables. Two principal components are got and the three strains with different characters are distinguished. At the same time, principal component 1 is found to be corresponded with arachidonic acid accumulation and principal component 2 is corresponded with biomass yield.

Key words: principal component analysis, arachidonic acid, Mortierella alpina, metabolomics, GC-MS analysis