食品科学 ›› 2008, Vol. 29 ›› Issue (7): 291-296.

• 生物工程 • 上一篇    下一篇

墨汁鬼伞液体发酵营养因子对菌丝体生长和胞外多糖产量影响的研究

毕华南,丁重阳,石贵阳,章克昌   

  1. 江南大学生物工程学院 工业生物技术教育部重点实验室; 江南大学生物工程学院; 工业生物技术教育部重点实验室; 江苏无锡214122
  • 出版日期:2008-07-15 发布日期:2011-07-28

Study on Effects of Nutritional Factors for Liquid Fermentation of Coprinus atramentarius on Mycelium Growth and Yield of Extracellular Polysaccharide

 BI  Hua-nan, DING  Zhong-Yang, SHI  Gui-Yang, ZHANG  Ke-Chang   

  1. School of Biotechnology, Jiangnan University, Key Laboratory of Industrial Biotechnology, Ministry of Education, Wuxi 214122, China
  • Online:2008-07-15 Published:2011-07-28

摘要: 本研究实现了墨汁鬼伞[Coprinus atramentarius(Bull.Fr.)Fr.]的液体培养,通过单因素试验研究了不同碳源、氮源及无机盐对墨汁鬼伞菌丝体生长和胞外多糖产量的影响。在此基础上,通过响应曲面法(RSM)研究了各营养因子的最佳水平,得到当葡萄糖、玉米粉、麸皮、KH2PO4和MgSO4·7H2O分别为13.51、17.07、7.62、2.29和2g/L时,胞外多糖最大预测值为293.73mg/L;当上述变量为10.07、18.02、11.63、1.58和4g/L时,菌丝体最大生长量为6.24g/L。

关键词: 墨汁鬼伞, 液体发酵, 胞外多糖, 生物量, 响应曲面法

Abstract: Coprinus atramentarius was cultivated with submerged fermentation method. Various nutritional factors including carbon sources, nitrogen sources and inorganic salt affecting the yield of extracellular polysaccharide (EPS) and mycelium growth were studied by single factor test. The key influential factors were further investigated by response surface methodology. By solving the quadratic regression model equation with appropriate statistic methods, the optimum concentrations for the sub-merged fermentation medium are determined that glucose, corn powder, wheat bran, KH2PO4 and MgSO·47H2O are 13.51, 17.07, 7.62, 2.29 and 2 g/L, respectively, and the predicted yield of the extracellular polysaccharide is 293.73 mg/L. When the above values are 10.07, 18.02, 11.63, 1.58 and 4 g/L respectively, the maximum biomass is 6.24 g/L.

Key words: Coprinus atramentarius, liquid fermentation, extracellular polysaccharide, biomass, response surface methodology