食品科学 ›› 2008, Vol. 29 ›› Issue (7): 345-347.

• 分析检测 • 上一篇    下一篇

槟榔及其产品中生物碱成分的反相离子对高效液相色谱法测定

张春江, 吕飞杰,台建祥,王祝年,付勤   

  1. 中国农业大学食品科学与营养工程学院; 中国农业科学院作物科学研究所; 中国热带农业科学院热带作物品种资源研究所; 中国农业科学院作物科学研究所 北京100083; 北京100081; 海南儋州571737
  • 出版日期:2008-07-15 发布日期:2011-07-28

Quantitative Determination of Alkaloids in Areca Nut and Its Products by RP-IP-HPLC

 ZHANG  Chun-Jiang, 吕Fei-Jie , TAI  Jian-Xiang, WANG  Zhu-Nian, FU  Qin   

  1. 1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2.Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3.Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China
  • Online:2008-07-15 Published:2011-07-28

摘要: 通过反相离子对高效液相色谱法测定了槟榔及其产品中的生物碱。采用HypersilBDSC18(250×4.6mm,5μm)色谱柱,柱温60℃;流动相为水-甲醇-磷酸-SDS(55ml∶45ml∶0.1ml∶1g),流速为1.0ml/min;采用SPD-20A紫外检测器,检测波长为215nm。结果显示,槟榔碱和槟榔次碱在25min内分离良好。测定的精密度为0.87%~1.58%,加标回收率为96.77%~100.23%。应用该方法对槟榔及其产品中槟榔碱和槟榔次碱的含量进行了测定分析。

关键词: 高效液相色谱, 槟榔, 槟榔碱, 槟榔次碱

Abstract: A method was developed for quantitative dtermination of arecoline and arecaidine in areca nut and its products by reversed-phase ion-pair high performance liquid chromatography (RP-IP-HPLC). Chromatographic separation was performed on Hypersil BDS C18 (250×4.6 mm, 5μm) column using a mixture (water: methanol: phosphoric acid: SDS=55 ml : 45 ml : 0.1 ml :1 g) as mobile phase with flow rate 1.0 ml/min and using SPD-20A ultraviolet detection at 215 nm. Arecoline and arecaidine are eluted within 25 min with satisfactory separation. Relative standard deviation of 0.84%~1.56% and average recovery of 96.77%~100.23% for the method are achieved. The contents of alkaloids in different areca nuts and their products were determinded by this method.

Key words: HPLC, areca nut, arecoline, arecaidine