食品科学 ›› 2008, Vol. 29 ›› Issue (4): 200-202.

• 工艺技术 • 上一篇    下一篇

响应曲面法优化葛根保健酒发酵工艺

 张丽杰, 赵天涛, 全学军, 陆天健   

  1. 重庆工学院生物工程学院; 重庆工学院生物工程学院
  • 出版日期:2008-04-15 发布日期:2011-08-24

Fermentation Optimization of Pueraria lobata Health Wine by Response Surface Methodology

 ZHANG  Li-Jie, ZHAO  Tian-Tao, QUAN  Xue-Jun, LU  Tian-Jian   

  1. College of Bioengieering, Chongqing University of Technology
  • Online:2008-04-15 Published:2011-08-24

摘要: 以葛根为原料,液化后采用糖化酶进行糖化,并应用活性干酵母为发酵剂生产葛根保健酒。利用响应曲面法对有交互作用的三个因素糖化酶、α-淀粉酶和酵母菌进行了优化。研究表明,当糖化酶的量为164.8μ/g原料,α-淀粉酶的量为90.08μg原料,酵母菌为1.041g,可得最大酒精度6.37%,最大总黄酮溶出量28.66mg/ml。

关键词: 葛根, 响应曲面法, 总黄酮

Abstract: The Pueraria lobata was used as the raw material, and glucoamylase used for saccharification after the liquefication, and active dry yeast used as ferment. Using response surface methodology (RSM), by which the interaction between the three factors: glucoamylase,α-aylase and active dry yeast,and their optimum levels were studied. The results showed that the highest alcohol concentration is 6.42 % and the highest flavone concentration is 28.69 mg/ml when the fermentation is carried out with glucoamylase concentration of 164.8U/g raw material, α-Amylase concentration of 90.08U/g raw material and active dry yeast concentration of 1.041 g.

Key words: Pueraria lobata, response surface methodology, flavone