食品科学 ›› 2008, Vol. 29 ›› Issue (8): 65-69.

• 基础研究 • 上一篇    下一篇

甜叶菊废弃物制得生物制剂的抗氧化活性研究

于慧,王锡昌,奚印慈   

  1. 上海海洋大学食品学院
  • 出版日期:2008-08-15 发布日期:2011-08-26

Study on Antioxidant Activity of Biological Preparation from Stevia rebaudiana Waste

 YU  Hui, WANG  Xi-Chang, XI  Yin-Ci   

  1. College of Food Science and Technology,Shanghai Ocean University,Shanghai 200090,China
  • Online:2008-08-15 Published:2011-08-26

摘要: 选择移植中国的南美甜叶菊在生产糖苷时剩余废弃杆茎等为原料,经不同工艺制得三种生物制剂(简称SC1、SC2、SC3),分别采用清除DPPH自由基法、氧自由基吸收能力(ORAC)法和过氧化值(POV)法研究其抗氧化能力。结果表明:三种生物制剂均具有一定的DPPH自由基清除能力,且随着浓度增加,其清除能力逐渐增强。在添加量0.015%时,生物制剂SC3的DPPH自由基清除能力可达92.21%,为BHT的1.1倍,VE的5.5倍;且其抗氧化稳定性也远强于BHT、VE和VC。另外,用ORAC法比较了SC3与天然鱼露的抗氧化能力,SC3的ORAC值高达326.28trolox μmol/ml,是天然鱼露的6.7倍。另外,鲐鱼松的贮藏实验表明了SC3以0.2%的添加量具有较好的抗氧化效果。研究表明SC3是一种具有很大开发价值的新型、天然、安全、高效的抗氧化生物制剂。

关键词: 甜叶菊, 生物制剂, 抗氧化能力

Abstract: With the trash stem as raw material,which was remained in the production process of glycoside from Stevia rebaudiana transplanted in China from South America,three biological preparations,namely Sc1,Sc2 and Sc3 were obtained by different processes. Their antioxidant activities were assessed by the method of 2,2-diphenyl-1-picryl hydrazyl(DPPH) radical assay,oxygen radical absorbance capacity(ORAC) assay and POV value. The results indicated that they have some certain DPPH radical scavenging activities,and the activities rise with the increase of concentration. When the addition amount of the Sc3 is 0.015%,its scavenging activity to DPPH radical reaches 92.21%,as 1.1 times as that of synthetic antioxidant BHT and as 5.5 times as that of natural antioxidant vitamin E,and especially its stability of the DPPH radical scavenging activity is better than that of BHT,vitamin E or vitamin C. Furthermore,the antioxidant activities of the Sc3 and natural fish sauce were assessed by the ORAC assay. Its ORAC value is 326.28 trolox μmol/ml,as 6.7 times as that of the natural fish sauce. Besides,the storage results of Scomber japonicus floss indicated that the Sc3 has good antioxidant effect at 0.2% addition level. So it is a novel,natural,safe and highly effective antioxidant biological preparation with great development value.

Key words: Stevia rebaudiana, biological preparation, antioxidant capacity