食品科学 ›› 2008, Vol. 29 ›› Issue (4): 291-295.

• 分析检测 • 上一篇    下一篇

芹菜提取物中芹菜素的HPLC与HPTLC定量分析研究

王克勤, 罗军武, 陈静萍, 陈亮   

  1. 湖南农业大学茶学教育部重点实验室; 湖南省原子能农业应用研究所; 湖南省原子能农业应用研究所;
  • 出版日期:2008-04-15 发布日期:2011-08-24

Study on Apigenin Quantitation Analysis from Celery Extractions by High-performance Liquid Chromatography (HPLC) and High-performance Lamina Chromatography (HPTLC)

 WANG  Ke-Qin, LUO  Jun-Wu, CHEN  Jing-Ping, CHEN  Liang   

  1. 1.Key Laboratory of Tea Science, Ministry of Education; 2. Hunan Institute of Atomic Energy Application in Agriculture
  • Online:2008-04-15 Published:2011-08-24

摘要: 通过采用高效液相色谱(HPLC)、紫外光谱和黄酮颜色特征反应对芹菜提取物主要成分芹菜素进行定性鉴定,同时建立定量测量芹菜素的高效薄层色谱(HPTLC)方法。结果表明:芹菜提取物的主要成分是黄酮醇-芹菜素,与芹菜素标准品具有相同的光谱和色谱性质;采用G60高效硅胶板,以氯仿-甲醇-水(18:2.3:0.35)为展开剂,芹菜素标样与试样有相同的Rf值为0.65;用最小二乘法作线性回归,芹菜素含量与斑点峰面积的标准曲线回归方程:y=15138x+7594.8,R2=0.993,线性范围:0.332~0.996μg/斑点。精密度实验RSD(n=5)=1.33%,平均回收率97.82%。

关键词: 高效液相色谱(HPLC), 高效薄层色谱(HPTLC), 芹菜素, Rf值

Abstract: By high-performance liquid chromatography (HPLC), UV spectrum and high-performance lamina chromatography (HPTLC) and color characteristic reaction to quantify the flavonoids, the basic components of thick celery extractions were identified as apigenin based on the quantitative analysis method with high-performance lamina chromatography (HPTLC). The results indicated that the main component of thick celery flavonoids is apigenin which has the same spectrum and chromatogram characteristics with the same Rf value(0.65) as the standard sample by the high-performance silica gel board G60 and the outspread solvent (chloroform:methanol:water, 18:2.3:0.35). The standard curve regress equation is: y=15138x +7594.8, R2=0.993 with linearity scope: 0.332 ~0.996μg per spot. The precision RSD(n=5) is 1.33 %, and the average callback rate is 97.82%.

Key words: high-performance liquid chromatography (HPLC), high-performance lamina chromatography (HPTLC), apigenin, Rf value