食品科学 ›› 2008, Vol. 29 ›› Issue (8): 184-187.

• 工艺技术 • 上一篇    下一篇

毛竹叶水溶性多糖BPS11的色谱研究

周跃斌,周向荣, 王伟   

  1. 湖南农业大学产业处; 湖南农业大学天然产物研究中心
  • 出版日期:2008-08-15 发布日期:2011-08-26

Study on Characteristics of Water Soluble Polysaccharide BPS11 Extracted from Phyllostachys pubescens Leaves by Chromatography

 ZHOU  Yue-Bin, ZHOU  Xiang-Rong, WANG  Wei   

  1. 1.Product Agent,Hunan Agricultural University,Changsha 410128,China;2.Hunan Standardization Institute,Changsha 410007,China;3.Research Center for Natural Products,Hunan Agricultural University,Changsha 410128,China
  • Online:2008-08-15 Published:2011-08-26

摘要: 将毛竹叶多糖粗提物通过柱色谱进行分离后得到BPS11,运用紫外扫描、薄层色谱、气相色谱对BPS11的理化性质进行了实验。结果表明,BPS11的理化性质与文献报道的结果相似;其单糖组成:未知单糖、鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖分别为0.50%、4.42%、13.90%、6.63%、11.49%、46.06%、17.00%,与文献报道结果不同。

关键词: 毛竹叶, 水溶性多糖, 柱色谱, 薄层色谱, 气相色谱

Abstract: The polysaccharides from Phyllostachys pubescens leaves were purified by DEAE-52 cellulose column chromatography and Sephadex G-75 glucan gel column chromatography successively,and a kind of polysaccharide named as BPS11 was obtained. In order to study the physicochemical characteristics of the polysaccharide,ultra violet spectroscopy(UV) ,thin-layer chromatography(TLC) and gas chromatography(GC) were employed. Results showed that the components of the polysaccharide include an unknown monosacchride,rhamnose,arabinose,xylose,mannose,glucose and galatose with contents of 0.50%,4.42%,13.90%,6.63%,11.49%,46.06% and 17.00%,respectively. These are remarkably different from data reported in the past literatures.

Key words: Phyllostachys pubescens leaves, water soluble polysaccharide, column chromatography, thin-layer chromatography, gas chromatography