食品科学 ›› 2008, Vol. 29 ›› Issue (2): 158-162.

• 工艺技术 • 上一篇    下一篇

蜂蛹黄酮的提取及体外抗氧化作用的研究

 张海生, 陈锦屏   

  1. 陕西师范大学食品工程系; 陕西师范大学食品工程系 陕西西安710062; 陕西西安710062;
  • 出版日期:2008-02-15 发布日期:2011-08-24

Study on Extraction Technology by Ultrasound and Antioxidation in vitro of Honeybee Pupa Flavonoids

 ZHANG  Hai-Sheng, CHEN  Jin-Ping   

  1. Department of Food Engineering, Shaanxi Normal University, Xi’an 710062, China
  • Online:2008-02-15 Published:2011-08-24

摘要: 研究了利用超声波技术对蜂蛹黄酮的辅助提取工艺及提取物的体外抗氧化作用。结果表明,超声波辅助提取蜂蛹黄酮时间短、效果好。最佳提取工艺条件为:提取溶剂为甲醇,超声波强度34W/cm2,超声波处理时间20min,料液比1:15。黄酮的一次提取量为43.85mg/100g。蜂蛹黄酮对碱性邻苯三酚自氧化产生的超氧阴离子、Fenton反应产生的羟基自由基和Fe2+诱发卵黄低密度脂蛋白(LDL)多不饱和脂肪酸(PUFA)过氧化产生的烷氧基(LO·)和烷过氧基(LOO·)均有明显的抑制作用,且均呈现一定的量效关系。

关键词: 蜂蛹, 黄酮, 超声波, 提取, 抗氧化

Abstract: The extraction technology of honeybee pupa flavonoides by ultrasound and it’s antioxidation in vitro were studied in this research. The results showed that it costs short time to extract honeybee pupa flavones by ultrasound with a good effect. The optimum extraction technology is ultrasound intensity 34 W/cm2, extraction time 20 min, ratio of material to solvent 1:15 with methanol as the solvent. The extraction amount of honeybee pupa was 43.85 mg/100 g. The honeybee pupa flavonoids have distinct inhibition against O2· made by alkaline pyrogallic acid, ·OH produced by Fenton reaction, LO·and LOO· peroxidation reaction of PUFA of yolk LDL induced by Fe2+, and dosage-effect correlations exist.

Key words: honeybee pupa, flavones, ultrasound, extraction, antioxidation