食品科学 ›› 2008, Vol. 29 ›› Issue (8): 258-260.

• 工艺技术 • 上一篇    下一篇

微波法提取黑曲霉抑菌成分的条件优化

李祝,郁建平,肖洋,梁宗琦, 李颖   

  1. 贵州大学生命科学院真菌资源研究所; 贵州大学生命科学院生化营养研究所; 贵州省产品质量检验检测院
  • 出版日期:2008-08-15 发布日期:2011-08-26

Optimization of Microwave-extration Conditions of Antibacterial Components from Aspergillus niger

 LI  Zhu, YU  Jian-Ping, XIAO  Yang, LIANG  Zong-Qi, LI  Ying   

  1. 1.Institute of Fungus Resource,College of Life Science,Guizhou University,Guiyang 550025,China;2.Institute of Biochemistry and Nutrition,College of Life Science,Guizhou University,Guiyang 550025,China;3.Institute of Products Detection and Determination of Guizhou Province,Guiyang 550003,China
  • Online:2008-08-15 Published:2011-08-26

摘要: 目的:优化黑曲霉发酵菌丝体中的抑菌成分微波提取法的最佳工艺。方法:以根癌农杆菌T-37作为指示菌,用滤纸片法测定提取液对T-37菌株的抑菌活性,通过正交试验优化微波提取抑菌成分的最佳条件。结果:优选出的最佳提取工艺为:20ml蒸馏水,固液比1:200,中火处理2.5min。结论:抑菌圈直径可达到40.86mm,该工艺简便、可行。

关键词: 微波提取, 黑曲霉, 正交试验, 抑菌圈

Abstract: Objective:To optimize the microvave-extraction procedure of antibacterial components from Aspergillus niger xj. Methods:Extraction procedure was screened by orthogonal test according to the inhibiting zones diameter,which was measured through filter paper method using Agrobacterium tumefaciens T-37 as indicator. Results:The optimized extraction conditions are as follows:distilled water 20 ml,solid-liquid ratio 1:200,and treating for 3 min with middle fire power of micro -wave. Conclusion :The inhibiting zone diameter reaches 40.86 mm. The procedure is very simple and feasible,which can be used as reference of production.

Key words: microvave-extraction, Aspergillus niger, orthogonal test, inhibiting zone