食品科学 ›› 2008, Vol. 29 ›› Issue (2): 225-228.

• 生物工程 • 上一篇    下一篇

柚苷酶产生真菌的复筛及菌种初步鉴定

 郭倩, 邬应龙, 黄高凌, 蔡慧农, 倪辉, 肖安风, 胡阳   

  1. 四川农业大学信息与工学院功能性食品实验室; 集美大学生物工程学院; 集美大学生物工程学院 四川雅安625014; 四川雅安625014; 厦门福建361021;
  • 出版日期:2008-02-15 发布日期:2011-08-24

Screening and Identification of Naringin Enzyme Producing Fungus for Debittering Pomelo Juice

GUO  Qian, WU  Ying-Long, HUANG  Gao-Ling, CAI  Hui-Nong, NI  Hui, XIAO  An-Feng, HU  Yang   

  1. 1. Functional Food Laboratory, Institute of Information and Engineering,Sichuan Agricultural University, Yaan 625014, China;2.School of Bioengineering, Jimei University, Xiamen 361021, China
  • Online:2008-02-15 Published:2011-08-24

摘要: 对前期分离获得的柚苷酶产生真菌进行复筛,从56株柚苷酶产生真菌中复筛出了8株脱苦能力较强的真菌,其中,实验室编号为4-4-1的菌株对柚皮苷的分解率为99%。对菌株4-4-1进行形态鉴定,结果表明,该真菌培养基中菌丝呈棉絮状,分生孢子梗单生,粗短,为轮辐状排列的瓶形小梗,分生孢子微弯、镰刀形,参照真菌鉴定手册第610页的描述鉴定为小型茄腐皮镰孢霉。

关键词: 柚苷酶, 真菌, 筛选, 鉴定, 蜜柚果汁

Abstract: By screening fungus producing naringin enzyme repeatedly 8 strains of superior debittering capability from 56 strains funguses were obtained. Results showed that No.4-4-1 laboratory’s strains was up to decomposition rate of 99% for naringin. No.4-4-1 strain’s conidiophore was single, tubbiness, and as bottle shape and ranging as spoke, and conidiophore peduncle was slightly bend as sickle. The hyphae were like cotton fibre in the culture medium. It was indentified as F.solani(Mart.) APP.et Wr.var.ninus Wr.according to the Fungal Identification Manual page 610.

Key words: naringin enzyme, fungus, screening, identification, juice of pomelo