食品科学 ›› 2008, Vol. 29 ›› Issue (2): 244-246.

• 生物工程 • 上一篇    下一篇

冷泡茶发酵菌种的诱变育种

 杨坚, 孙永康, 潘科   

  1. 西南大学食品科学学院; 西南大学食品科学学院 重庆400716; 重庆400716;
  • 出版日期:2008-02-15 发布日期:2011-08-24

Mutagenesis and Breeding of Fermentation Strains about Cold Water Infused Tea

 YANG  Jian, SUN  Yong-Kang, PAN  Ke   

  1. College of Food Science, Southwest University, Chongqing 400716, China
  • Online:2008-02-15 Published:2011-08-24

摘要: 对从茶叶中筛选出的1株纤维分解菌SW3进行紫外线和亚硝酸钠诱变,用刚果红透明圈平板法来进行初筛,再采用摇瓶复筛,从中筛选出发酵液滤纸酶活力高的突变株SW355。突变株SW355的发酵液滤纸酶活力比出发菌株SW3提高了62.9%,且其遗传性状稳定。

关键词:  , 纤维素酶; 诱变; 紫外线; 亚硝酸钠;

Abstract: A cellulase-decomposing bacterium SW3 was screened out from tea, and mutagenized with UV and NaNO2. The first isolation was conducted according to the method of distinct zone of clearing in cellulase-congo red agar medium. The second isolation was conducted according to the test of filter paper enzyme activity through liquid fermentation. SW355 is 62.9% higher than the original strain SW3, and it has a stable property in transmissibility.

Key words: cellulase, mutation, UV, NaNO2