食品科学 ›› 2008, Vol. 29 ›› Issue (8): 318-322.

• 工艺技术 • 上一篇    下一篇

榛子蛋白质提取及功能特性研究

马勇,张丽娜, 齐凤元,郑岩   

  1. 渤海大学生物与食品科学学院
  • 出版日期:2008-08-15 发布日期:2011-08-26

Study on Extraction of Hazelnut Protein and Its Functional Properties

 MA  Yong, ZHANG  Li-Na, QI  Feng-Yuan, ZHENG  Yan   

  1. College of Biology and Food Science,Bohai University,Jinzhou 121000,China
  • Online:2008-08-15 Published:2011-08-26

摘要: 采用碱提酸沉法从脱脂榛子粉中提取榛子蛋白质,研究浓度、温度等影响因素与榛子蛋白质功能特性的关系,并测定榛子蛋白质等电点。结果显示,从脱脂榛子粉中提取蛋白质最佳条件为:料液比1:6,碱溶pH8.0、温度40℃、时间0.5h,酸沉pH4.5;榛子蛋白质起泡性在浓度为4%时最高,泡沫稳定性在浓度为2%时最大;其乳化性在浓度为2%时最大,静置的温度对乳化稳定性影响很小;蛋白质吸油性为3.72ml/g;其蛋白质分散指数(PDI)在50℃时最大,50℃以上PDI开始下降,70℃以后变化不大;其在浓度1%、60℃、1.5h条件下持水性最好;其等电点为4.52。

关键词: 榛子蛋白粉, 提取条件, 功能特性, 等电点

Abstract: In the alkali extraction and acid precipitation process of protein from hazelnut,effects of concentration and temperature on the functional properties of the protein were investigated,and its isoelectric point was determined. The optimal preparation conditions of the protein are as follows:solid-liquid ratio 1:6,40 ℃ alkali extraction at pH 8.0 for 0.5 h and acid precipitation at pH 4.5. Its foamability is the highest at the concentration of 4%,with the highest foam stability and emulsibility at the concentration of 2%. The emulsion stability is hardly affected by standing temperature. The oil adsorption ability is 3.72 ml/g. The protein dispersibility index(PDI) is the highest at 50 ℃,while the PDI value decreases above 50 ℃,keeping stable above 70 ℃. The water holding capacity attains the highest on the conditions of 1% concentration,60 ℃ and 1.5 hours. Its isoelectric point is measured to be 4.52.

Key words: hazelnut protein, extraction condition, functional properties, isoelectric point