食品科学 ›› 2008, Vol. 29 ›› Issue (2): 280-283.

• 生物工程 • 上一篇    下一篇

肋脉羊肚菌液体培养条件的研究

 杨芳, 王新风, 朱骏, 李刚, 刘艳文, 袁生   

  1. 南京师范大学生命科学学院微生物工程重点实验室; 淮阴师范学院生物系资源微生物研究所; 南京师范大学生命科学学院微生物工程重点实验室 江苏南京210097 淮阴师范学院生物系资源微生物研究所; 江苏淮安223300; 江苏南京210097;
  • 出版日期:2008-02-15 发布日期:2011-08-24

Study on Liquid Cultivating Conditions of Morchella costata (Vent.) Pers.

 YANG  Fang, WANG  Xin-Feng, ZHU  Jun, LI  Gang, LIU  Yan-Wen, YUAN  Sheng   

  1. 1.Key Laboratory of Microbial Engineering, College of Life Sciences, Nanjing Normal University, Nanjing 210097, China;2.Institute of Resource Microorganism, Department of Biology, Huaiyin Teachers’ College, Huaian 223300, China
  • Online:2008-02-15 Published:2011-08-24

摘要: 对肋脉羊肚菌进行液体摇瓶培养,以菌丝干重、胞内多糖产量及其得率为筛选指标,得到最适合肋脉羊肚菌生长的培养基条件为可溶性淀粉浓度2%、豆粕粉浓度1%、MgSO4·7H2O0.1%、KH2PO40.05%、初始pH7.5、培养温度28℃。培养8d后菌丝干重达16.94g/L,胞内多糖量达0.984g/L。

关键词: 肋脉羊肚菌, 液体培养, 胞内多糖

Abstract: The optimization of culture of Morchella costata (Vent.) Pers on liquid culture was studied. With dry weight of mycelium, inopolysaccharide(IPS) output and its yield. The results showed that the concentration of starch was 2%, the chorma of lobster sauce was 1%, the concentration of MgSO4·7H2O was 0.1% and the concentration of KH2PO4 was 0.05%, the initial pH of culture was 7.5, and the temperature was 28 ℃. The dry weight of mycelium reached 16.94 g/L, and the weight of inopolysaccharides(IPS) reached 0.984 g/L after culturing for 8 days.

Key words: Morchella costata (Vent.) Pers, liquid culture, inopolysaccharides(IPS)