食品科学 ›› 2008, Vol. 29 ›› Issue (4): 347-351.

• 分析检测 • 上一篇    下一篇

茶叶中γ-氨基丁酸提取、分离及薄层扫描测定的研究

郑红发, 黄亚辉, 黄怀生, 粟本文, 袁英芳   

  1. 湖南省茶叶研究所; 华南农业大学园艺学院; 湖南省茶叶研究所;
  • 出版日期:2008-04-15 发布日期:2011-08-24

Study on Extraction and Determination of γ-Aminobutyric Acid in Tea

 ZHENG  Hong-Fa, HUANG  Ya-Hui, HUANG  Huai-Sheng, SU  Ben-Wen, YUAN  Ying-Fang   

  1. 1.Huan Tea Research Institute; 2.College of Horticulture, South China Agricultural University
  • Online:2008-04-15 Published:2011-08-24

摘要: 对茶叶中γ-氨基丁酸的提取、分离及检测方法进行了研究,分别采用水和乙醇作为提取溶剂,结果发现用水提取能达到更好的效果,γ-氨基丁酸含量提高20%;同时采用离子交换树脂对γ-氨基丁酸提取液进行分离,提取液上732阳离子树脂柱(2.5×50cm),使用柠檬酸和氨水缓冲液进行pH线性梯度洗脱,洗脱液中γ-氨基丁酸最高浓度达39%;采用薄层扫描仪对γ-氨基丁酸的含量进行检测,研究并确定了检测的条件及参数。

关键词: &gamma, -氨基丁酸, 提取, 分离, 测定

Abstract: The method has been studied to extractisolate and determine the γ-aminobutyric acid in tea. Taking water or alcohol as solvent, it was discovered that water can achieve a better effect with, γ-aminobutyric acid content to be enhanced 20 %. With the ion exchange resin for isolation and 732 positive ions resin columns (2.5×50 cm) for adsorption, and citric acid and ammonia water as buffer liquids, so as to carry on pH linear gradient elution the results indicated that the γ-aminobutyric acid concentration can reach roughly 39 %. By thin layer scanner to continue the mensuration, the assaying conditions and parameters can be determined.

Key words: &gamma, -aminobutyric acid; extract; separate; determine;