食品科学 ›› 2008, Vol. 29 ›› Issue (5): 87-89.

• 基础研究 • 上一篇    下一篇

大孔吸附树脂纯化山茱萸总皂苷的动态洗脱条件研究

 徐建国, 田呈瑞, 胡青平   

  1. 山西师范大学食品工程系; 山西师范大学食品工程系;
  • 出版日期:2008-05-15 发布日期:2011-08-26

Study on Dynamic Desorption Conditions of Total Saposins from Cornus officinalis by Macroporous Resins

 XU  Jian-Guo, TIAN  Cheng-Rui, HU  Qing-Ping   

  1. Departerment of Food Engineering,Shanxi Normal University
  • Online:2008-05-15 Published:2011-08-26

摘要: 运用单因素试验和正交试验研究了山茱萸总皂苷在HPD-300大孔吸附树脂上的动态洗脱工艺,经极差分析和方差分析确定了其最佳操作条件。结果表明,最佳蒸馏水洗脱条件为:水洗流速1.0BV/h,用水量2.2BV,水洗时间2.2h;最佳洗脱剂洗脱条件为乙醇浓度50%,洗脱流速2.0BV/h,洗脱剂用量4.5BV。

关键词: 山茱萸, 总皂苷, 大孔吸附树脂, 动态洗脱

Abstract: This experiment studied dynamic de-adsorption technology of total saposins from Cornus officinalis in HPD-300 macroporous resin by single factor tests and orthogonal test designs.The optimal manipulation conditions were ascertained by variance analyses.The results showed that the optimum desorption conditions are:flowing rate 1.0 BV/h,volume 2.2 BV,and desorption time 2.2 h;The best desorption conditions by alcohol are 50% concentration,flowing rate 2.0 BV/h,and desoprtion volume 4.5 BV.

Key words: Cornus officinalis, total saposins, macroporous resin, dynamic desorption