食品科学 ›› 2008, Vol. 29 ›› Issue (5): 112-115.

• 基础研究 • 上一篇    下一篇

L-赖氨酸与D-核糖的模式美拉德反应及其产物抗氧化性能研究

王惠英, 孙涛, 周冬香, 毛芳   

  1. 上海水产大学食品学院; 上海水产大学食品学院;
  • 出版日期:2008-05-15 发布日期:2011-08-26

Study on Model Maillard Reaction of L-lysine and D-ribose and Antioxidant Activity of Its Products

 WANG  Hui-Ying, SUN  Tao, ZHOU  Dong-Xiang, MAO  Fang   

  1. College of Food Science and Technology,Shanghai Fisheries University
  • Online:2008-05-15 Published:2011-08-26

摘要: 研究L-赖氨酸与D-核糖的模式美拉德反应(L-赖氨酸的氨基与D-核糖的羰基的物质量之比分别为1:1、1:2和2:1),考察了三个体系反应过程中产物pH值、吸光度的变化,并以金属螯合能力、超氧阴离子自由基(O-2·)清除能力、二苯代苦味酰自由基(DPPH·)清除能力以及还原能力为指标,评价了美拉德反应产物(MRPs)的抗氧化效果。结果表明:美拉德反应是个酸度和褐变不断增强的反应;MRPs的最大吸收波长在460nm左右;达到相同褐变程度,即吸光度(A)为2.2时,1:2的体系最快,其次是1:1的体系,2:1的体系相对最慢。MRPs有较好的金属螯合能力,可有效清除O-2·和DPPH·自由基,但反应终产物几乎没有还原能力。

关键词: 美拉德反应, 金属螯合, O-2·, DPPH·, 还原能力, 抗氧化能力

Abstract: The Maillard reaction products(MRPs) were prepared using L-lysine and DMaillard reaction.The pH value and absorbance of the Maillard reaction products(MRPs) were determined.In this research,the antioxidative activities of MRPs,including iron-chelation activity,scavenging effect on superoxide anion radical and DPPH· radical,and reducing power were studied.The results showed that browning and acidity increased with the heating time;the wavelength of maximal absorbance was about 460 nm;While reaching the same browing degree,namely absorbance 2.2,their reaction speeds were shown as follows:1:2>1:1>2:1.Three kinds of MRPs with different proportions of amido and carbonyl exhibited good iron-chelation activities and scavenging activities against superoxide anion radical and DPPH· radical,but they almost did not have reducing powers.

Key words: Maillard reaction; iron-chelation; superoxide anion; 1,1-diphenyl-2-picrylhydrazyl(DPPH·, ); reduc-ing power; antioxidant activity;