柑桔皮总黄酮提取工艺优化及其数学模型研究

食品科学 ›› 2008, Vol. 29 ›› Issue (5): 167-172.

柑桔皮总黄酮提取工艺优化及其数学模型研究

苏东林, 单杨, 李高阳, 张菊华, 张群, 周向荣

湖南省农产品加工研究所；湖南省标准化研究院；湖南省柑桔工程技术研究中心；湖南省柑桔工程技术研究中心；

出版日期:2008-05-15 发布日期:2011-08-26

Study on Optimization of Total Flavonoids Extraction Technology from Citrus Peel and Its Mathematical Model

SU Dong-Lin, DAN Yang, LI Gao-Yang, ZHANG Ju-Hua, ZHANG Qun, ZHOU Xiang-Rong

1.Hunan Agricultural Product Processing Institute;2.Centre for Citrus Engineering Technology Research in Hunan;3.Hunan Standardization Institute

Online:2008-05-15 Published:2011-08-26

摘要/Abstract

摘要： 为充分利用农业废弃物柑桔皮,本实验采用二次通用旋转组合设计研究了柑桔皮中总黄酮的超声波辅助提取工艺,并对建立的数学模型进行了规划求解及验证实验。结果表明:pH值对总黄酮得率有极显著影响,乙醇浓度、液料比以及时间同乙醇浓度之间的和时间同pH值之间的交互项对总黄酮得率有显著影响,其他因素对总黄酮得率无显著影响。经实验验证,当pH值为5.6、乙醇浓度为75%、液料比为26ml/g、超声时间为80min时总黄酮得率最高,达3.314%±0.063%(n=3),与理论计算值3.348449%基本一致。回归模型可较好地预测柑桔皮中总黄酮的提取得率。

关键词: 柑桔皮, 总黄酮, 超声波辅助提取, 数学模型, 响应面法

Abstract: In order to make full use of agricultural waste material—citrus peel,ultrasonic wave-assisted extraction technique of total flavonoids from citrus peel was studied according to a current,rotational and combinational design of quadratic regression.Then,one quadratic regression model for the total flavonoids yield was established.The extraction conditions were optimized by the above model,while the optimized results were verified by experiments.Results showed that the pH value has a very significant effect on the extraction rate of total flavonoids.The ethanol concentration,ratio of solution to material,interaction between time and ethanol concentration,and time and pH value show significant effects on the extraction rate.Other factors have no significant effects on the extraction rate.Research results indicated that the optimized extraction conditions for maximum yield of total flavonoids are:pH value 5.6,ethanol concentration 75%,ratio of solution to material 26 ml/g,and extraction time 80 min.Under the optimized conditions,the experimental value of yield is 3.314%±0.063%(n=3),close to the estimated value 3.348449% attained by regression model.The model for the total flavonoids yield have better prediction effects.

Key words: citrus peel, total flavonoids, ultrasonic wave-assisted extraction, mathematical model, response surface methodology(RSM)

苏东林, 单杨, 李高阳, 张菊华, 张群, 周向荣. 柑桔皮总黄酮提取工艺优化及其数学模型研究[J]. 食品科学, 2008, 29(5): 167-172.

SU Dong-Lin, DAN Yang, LI Gao-Yang, ZHANG Ju-Hua, ZHANG Qun, ZHOU Xiang-Rong. Study on Optimization of Total Flavonoids Extraction Technology from Citrus Peel and Its Mathematical Model[J]. FOOD SCIENCE, 2008, 29(5): 167-172.

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