食品科学 ›› 2008, Vol. 29 ›› Issue (5): 173-176.

• 工艺技术 • 上一篇    下一篇

啤酒废酵母中谷胱甘肽提取工艺条件优化研究

 邵伟, 乐超银, 唐明, 熊泽   

  1. 三峡大学化学与生命科学学院; 三峡大学化学与生命科学学院;
  • 出版日期:2008-05-15 发布日期:2011-08-26

Study on Optimization of Extraction Technique of Glutathione from Beer Yeast Waster with Response Surface Methodlogy

 SHAO  Wei, LE  Chao-Yin, TANG  Ming, XIONG  Ze   

  1. College of Chemistry and Life Science,Three Gorges University
  • Online:2008-05-15 Published:2011-08-26

摘要: 以啤酒废酵母为原料,通过单因素试验从料水比、提取时间、提取温度和醋酸添加量等四个因素中确定了三个主要因素,并根据Box-Benhnken的中心组合实验设计原则,应用响应面法对谷胱甘肽(GSH)提取工艺参数进行了分析,得到了谷胱甘肽提取的最佳工艺条件:在料水比1:4,温度43.6℃的条件下,添加3.5%的醋酸,提取5.5h。此条件下谷胱甘肽的提取得率可达到29%以上。

关键词: 啤酒废酵母, 谷胱甘肽, 提取, 响应面

Abstract: With beer yeast waster as the raw material,the important effect factors such as among ratio of material to water,time,temperature and dosage of acetic acid were confirmed by the single factor tests primarily,then according to the Box-Benhnken center combined experimental design principles,the 3 most important effect factors were analyzed with RSM.The optimum conditions were obtained as follows:ratio of material and water 1:4,temperature 43.6 ℃,dosage of acetic acid 3.5%,and time 5.5 h.Under these conditions,the content of GSH red reach over 29%.

Key words: beer yeast waste, glutathione(GSH), extraction, response surface methodlogy(RSM)