食品科学 ›› 2008, Vol. 29 ›› Issue (5): 177-180.

• 工艺技术 • 上一篇    下一篇

超滤分离纯化麻竹叶黄酮的研究

 唐浩国, 李叶, 向进乐, 徐宝成, 魏晓霞, 陈宗道   

  1. 河南科技大学食品与生物工程学院; 西南大学食品科学学院;
  • 出版日期:2008-05-15 发布日期:2011-08-26

Study on Purification of Flavonoids from Leaves of Dendrocalamus latiflorus by Ultrafiltration

 TANG  Hao-Guo, LI  Ye, XIANG  Jin-Le, XU  Bao-Cheng, WEI  Xiao-Xia, CHEN  Zong-Dao   

  1. 1.College of Food and Bioengineering,Henan University of Science and Technolygy;2.College of Food Science,Southwest University
  • Online:2008-05-15 Published:2011-08-26

摘要: 通过对5种超滤膜的对比研究,选择出切割分子量为50000D的聚砜膜PSF-500对麻竹叶黄酮有较好的分离纯化效果:产品得率高达4.29%,纯度达到44.132%。进而选择运行压力、进料液流速和运行时间等运行参数测定了聚砜膜PSF-500的超滤效果,确定了最佳工艺参数:采用操作压力0.20~0.30MPa,进料液流速0.4m/s,连续运行12h清洗超滤膜。还对聚砜膜PSF-500超滤系统进行了芦丁加样回收率测试,结果表明平均回收率为97.0%,变异系数为1.94%。

关键词: 麻竹叶, 黄酮, 超滤, 聚砜膜

Abstract: The PSF-500 of polysulfone membrane with a molecular weight cut-off(MWCO) of 50000 had the best effect on purification of flavonoids from leaves of Dendrocalamus latiflorus by ultrafiltration(UF) in contrast with five other membranes.The yield of flavonoids is 4.29 % and the content is 44.123 %.The effects of filtration pressure,feed velocity of flow and runtime on the operation were tested and discussed.The results showed that the best parameters on the operation were filtration pressure 0.20~0.30 MPa,feed velocity of flow 0.4 m/s and runtime 12 h(the PSF-500 membrane must be cleaned after 12 h).At the same time,the recovery test of adding rutin was experimented with PSF-500 in UF system.The results indicated that average recovery is 97.0% and coefficient of variation is 1.94%.

Key words: leaves of Dendrocalamus latiflorus, flavonoids, ultrafiltration, polysulfone membrane