食品科学 ›› 2008, Vol. 29 ›› Issue (5): 227-230.

• 工艺技术 • 上一篇    下一篇

罗非鱼鱼皮多肽的超滤分离及其抗氧化活性研究

 刘成梅, 梁汉萦, 刘伟, 万婕   

  1. 南昌大学食品科学教育部重点实验室 中德食品工程中心; 南昌大学食品科学教育部重点实验室; 中德食品工程中心;
  • 出版日期:2008-05-15 发布日期:2011-08-26

Study on Separation of Peptides Derived from Oreochromis niloticus Skin with Ultra-filtration Technology and Its Antioxidative Activity

 LIU  Cheng-Mei, LIANG  Han-Ying, LIU  Wei, WAN  Jie   

  1. Key Laboratory of Food Science,Ministry of Education,Nanchang University,Sino-German Food Engineering Center
  • Online:2008-05-15 Published:2011-08-26

摘要: 采用超滤技术分离罗非鱼鱼皮蛋白酶解液(TSP-I),研究超滤压力、时间和浓度对膜渗透通量、渗透增量和膜效能的影响。结果表明:超滤处理的压力为28Psi,时间为40min,浓度为5%的操作条件下,膜渗透通量为9.98LHM,渗透增量为2.68g/10min,膜效能为3.41g/m2·min,超滤处理达最佳效果。利用分光光度法检测了抗坏血酸和超滤后所得多肽粉(TSP-II)对超氧阴离子自由基(O2·)、羟基自由基(·OH)、二苯代苦味酰自由基(DPPH·)及双氧水(H2O2)的清除能力。实验发现:超滤处理所得多肽具有一定的抗氧化能力,TSP-II对四种自由基的清除作用顺序为:H2O2>·OH>O2·>DPPH·。以清除H2O2的能力为代表相比较,0.80mg/mlTSP-II和0.03mg/ml抗坏血酸清除H2O2的能力相当。

关键词: 罗非鱼鱼皮, 胶原多肽, 抗氧化作用, 分光光度法

Abstract: Peptides derived from TSP-I was separated by ultra-filtration technology,the effects of pressure,temperature and concentration of peptides on ultra-filtration permeate flux,permeate increment and membrane efficiency.The results showed that the optimum conditions are:5% TSP-I,pressure 28 Psi and time 40 min.Under the condition,ultra-filtration permeate flux is 9.98 LHM,ultra-filtration permeate increment is 2.68 g/10min and membrane efficiency is 3.41 g/m2·min.The capacities on scavenging O2·,·OH,DPPH·and H2O2 of VC and TSP-II were investigated by spectrophotometry.Study informed that TSP-II had a certain extent antioxidation,the order of scavenging ability is H2O2>·OH >O·2>DPPH·.On scavenging H2O2,0.80 mg/ml TSP-II is equivalent to 0.03 mg/ml VC.

Key words: tilapia(Oreochromis niloticus)skin, collagen polypeptide, radicalanti-oxidizing action, spectrophotometry