食品科学 ›› 2008, Vol. 29 ›› Issue (5): 231-233.

• 工艺技术 • 上一篇    下一篇

小黑豆多肽加工工艺的研究

 王常青, 任海伟   

  1. 山西大学生命科学与技术学院; 山西大学生命科学与技术学院;
  • 出版日期:2008-05-15 发布日期:2011-08-26

Study on Preparation Technology of Small Black-soybean Peptide

 WANG  Chang-Qing, REN  Hai-Wei   

  1. Institute of Life Science and Technology,Shanxi University
  • Online:2008-05-15 Published:2011-08-26

摘要: 本研究采用双酶分步水解法制备小黑豆多肽。最佳工艺条件为:先用枯草杆菌碱性蛋白酶,在50℃、pH9.0下水解经预处理的小黑豆分离蛋白4h;再用霉菌中性蛋白酶在pH7.0、50℃水解4h,加酶量均为4000U/g。该工艺的多肽得率可以达到80%以上,水解度(DH%)为32.75%。凝胶色谱分析表明,小于10000D的多肽可达到总多肽的91.61%。

关键词: 小黑豆多肽, 双酶水解, 多肽得率, 分子量分布, 水解度

Abstract: The optimum conditions about sequentially hydrolyzing small black-soybean protein isolated were:the small black-soybean were hydrolyzed by alkaline proteinases in the condition of pH 9.0,50 ℃ for 4 hours,and then,hydrolyzed continuously by neutral protease in the condition of pH7.0,50 ℃ for 4 hours,and the quantity of pronase is 4000U/g.The peptide yield reached 80%,and the degree of hydrolysis(DH) of black-soybean protein isolate is 32.75%.The result of gel chromatography showed that the percentage of the component of black-soybean peptides with a molecular weight below 10000 D 91.61 %.

Key words: small black-soybean peptide, double enzymolysis, peptide yield, molecular weight distribution, DH