食品科学 ›› 2008, Vol. 29 ›› Issue (5): 265-268.

• 生物工程 • 上一篇    下一篇

二级连续系统混合培养益生菌及纤维益生菌片的研制

薛胜平, 杜连祥, 路福平   

  1. 河北经贸大学生物科学与工程学院; 天津工业微生物重点实验室 天津科技大学生物技术学院; 天津工业微生物重点实验室; 天津科技大学生物技术学院;天津工业微生物重点实验室;
  • 出版日期:2008-05-15 发布日期:2011-08-26

Two Stages Continuous Fermention Process of Five Probiotic Bacterial Strains for Fiber Embeded Probiotic Tablets

 XUE  Sheng-Ping, DU  Lian-Xiang, LU  Fu-Ping   

  1. 1.College of Biosicence and Bioengineering,Hebei Economic and Business University;2.Tianjin Key Laboratory of Industrial Microbiology,College of Biotechnology,Tianjin University of Science and Technology
  • Online:2008-05-15 Published:2011-08-26

摘要: 将填充床固定化系统连于单级液态连续培养系统后构建二级连续培养系统,接种肠膜芽孢杆菌、双歧杆菌与酪酸菌、嗜酸乳杆菌、粪肠球菌,运行12d、三个批次,未出现污染、堵塞现象,五种益生菌固相总生物量高达3.5×1010/g。连续混合培养五种益生菌,菌密度高,菌与纤维载体不需分离,经干燥、干法压片得到了纤维益生菌片,产品活菌数为109/g,高出国家标准1000倍。

关键词: 固定化, 连续培养, 混合培养, 益生菌, 纤维益生菌片

Abstract: Five strains of probiotic bacteria were fiber embeded and cultured in a continuous fibrous bed bioreactor with corn fiber as the carrier.The immobilized biomass was studied by selective culture methods.Bifidobacterium longum TQ21-2-2,Lactobacillus acidophile CICC06005,Clostridum butyric TO-A,Bacillus mesentericus TO-A and Enterococcus faecalis T-110 were found to have coexisted stably.The total biomass of five probiotics in immobilized phase was 3.5×1010 g in corn fiber of the fibrous bed bioreactor.The system can be used to obtain formulation of microecological and fiber embeded tablets,so as to produce solid high biomass commensal probiotics products continuously.The fiber and bacteria were dried into functional food tablets with probiotics biomass in the amount of 109/g,1000 times higher than that estimated in China national standard.

Key words: immobilization, continuous culture, mixed culture, probiotics, food tablet