食品科学 ›› 2008, Vol. 29 ›› Issue (5): 303-306.

• 生物工程 • 上一篇    下一篇

右旋糖酐蔗糖酶生产菌的产酶条件研究

 张洪斌, 王雅洁, 胡雪芹, 朱慧霞, 姚日生   

  1. 合肥工业大学化工学院 农产品生物化工教育部工程研究中心; 合肥工业大学化工学院; 农产品生物化工教育部工程研究中心;
  • 出版日期:2008-05-15 发布日期:2011-08-26

Study on Producing Enzyme Conditions for Dextransucrase-producing Microorganism

 ZHANG  Hong-Bin, WANG  Ya-Jie, HU  Xue-Qin, ZHU  Hui-Xia, YAO  Ri-Sheng   

  1. School of Chemical Engineering,Hefei University of Technology,Engineering Research Center of Bio-Process,Ministry of Education
  • Online:2008-05-15 Published:2011-08-26

摘要: 右旋糖酐蔗糖酶是一种以蔗糖为底物,催化转移D-葡萄糖基生成α-葡聚糖或低聚糖的葡萄糖基转移酶。本实验对右旋糖酐蔗糖酶生产菌的产酶条件进行了研究。结果表明:诱导产酶碳源为5%蔗糖、培养时间24h、培养温度25℃、培养液初始pH值8.0为最佳产酶条件。

关键词: 右旋糖酐蔗糖酶, 蔗糖, 肠膜状明串珠菌, 产酶

Abstract: The dextransucrase is a kind of glucosyltransferases that can catalyze the transfer of D-glucopyranosyl units by synthesizing sucrose to α-glucans or oligosaccharides.In this research,enzyme producing conditions for dextransucrase-producing organism were studied.The results showed that the optimum conditions of producing dextransucrase are as follows:most suitable carbon source 5% sucrose,cultivating time 24 h,cultivating temperature 25 ℃ and cultivating solution initial pH value 8.0。

Key words: dextransucrase, sucrose, Leuconstoc mesenteriodes, enzyme production