食品科学 ›› 2008, Vol. 29 ›› Issue (5): 337-340.

• 生物工程 • 上一篇    下一篇

南昆山毛叶茶cDNA文库构建

 文海涛, 陈忠正, 赵亮, 郭健, 李斌, 蒋跃明   

  1. 华南农业大学食品学院; 浙江工商大学食品、生物与环境工程学院;
  • 出版日期:2008-05-15 发布日期:2011-08-26

Construction of cDNA Library of Camellia ptilophylla Chang

 WEN  Hai-Tao, CHEN  Zhong-Zheng, ZHAO  Liang, GUO  Jian, LI  Bin, JIANG  Yue-Ming   

  1. 1.College of Food Science,South China Agricultural University,Guangzhou 510642,China;2.College of Food Science,Biotechnology and Environmental Engineering,Zhejiang Gongshang University
  • Online:2008-05-15 Published:2011-08-26

摘要: 本研究以天然低咖啡碱茶树品种—南昆山毛叶茶春季嫩稍为材料,采用Stratagene公司ZAP系列试剂盒成功构建了该品种全长cDNA文库。原始文库滴度为6.0×105pfu/ml,重组率为97.3%,1.0~2.0kb的插入片段占整个文库的70%左右,达到一个高质量cDNA文库的要求,文库经扩增后滴度为1.9×109pfu/ml,重组率达到99.1%。

关键词: 南昆山毛叶茶, cDNA文库, 文库滴度

Abstract: In this study,cDNA library was constructed successfully from the specie of Camellia ptilophylla Chang with a low content of caffeine through using a series of ZAP kits that bought from the company of Stratagene.The titer and recombinant rate of this library were 6.0×105pfu/ml and 97.3 % respectively,the insert size with 1.0~2.0kb occupied 70% of the whole library,matching the requirement of high quality library.The titer and recombinant rate of amplification library were 1.9×109pfu/ml and 99.1%,respectively.

Key words: Camellia ptilophylla Chang, cDNA library, library titer