食品科学 ›› 2008, Vol. 29 ›› Issue (5): 344-366.

• 分析检测 • 上一篇    下一篇

毛细管电泳测定蘑菇中多种水溶性维生素

 黄劲松, 陈建兵, 杜先锋, 司雄元, 郑海敏   

  1. 池州学院化学与食品科学系; 安徽农业大学食品系; 安徽农业大学生物中心; 安徽农业大学园艺学院
  • 出版日期:2008-05-15 发布日期:2011-08-26

Capillary Electrophoretic Determination of Water-soluble Vitamins in Mushroom Powder

 HUANG  Jin-Song, CHEN  Jian-Bing, DU  Xian-Feng, SI  Xiong-Yuan, ZHENG  Hai-Min   

  1. 1.Department of Chemisty,Chizhou College;2.Department of Food Science,Anhui Agricultural University;3.Biotechnology Center,Anhui Agricultural University;4.School of Horture,Anhui Agricultural University
  • Online:2008-05-15 Published:2011-08-26

摘要: 建立了毛细管电泳法分析蘑菇粉中水溶性维生素的方法,并研究缓冲溶液、工作电压等因素对维生素测定的影响。样品进行测定时,先过C18柱,可以对样品中的维生素起到纯化和富集的作用。电泳条件为20kV,在20mmol/LpH8.72硼砂-硼酸缓冲液,10min内实现了蘑菇粉中多种水溶性维生素(VB1、VB2、VB6、VC和VPP)的有效分离,具有良好的稳定性、精密度、线性关系等。

关键词: 蘑菇粉, 高效毛细管电泳, 维生素

Abstract: A rapid capillary electrophoresis method was established for detecting water-soluble vitamins in mushroom powder.Effects of buffer solution and voltage on the separation of vitamins from mushroom powder were studied.The effective ingredients of the samples were purified and enriched by means of a solid-phase extraction step with a pre-conditioned C18 cartridge,and the compounds were eluted with pH4.2 HCl solution.VB1,VB2,VB6,VC and VPP could be separated on a 50 cm ×75 μm capillary column with 20 mmol/L borate buffer solution(pH8.72),at temperature 25 ℃ and electrophoresis voltage 20 kV,within 10 min,and the satisfied results were obtained with different characteristics including linearity,precision and stability.

Key words: mushroom powder, Capillary zone electrophoresis, water-soluble vitamins