食品科学 ›› 2008, Vol. 29 ›› Issue (5): 352-354.

• 分析检测 • 上一篇    下一篇

超高效液相色谱-串联质谱法快速分析草莓及其制品中杀虫双残留量

 卢晓宇, 王金花, 陈跃, 黄梅, 周琦, 徐超一   

  1. 北京出入境检验检疫局食品安全检测中心; 北京化工大学理学院; 北京出入境检验检疫局食品安全检测中心
  • 出版日期:2008-05-15 发布日期:2011-08-26

Method for Rapid Detection of Bisultap Residues in Strawberry and Its Products Using Ultra Performance Liquid Chromatography with Electrospray Tandem Mass Spectrometry

 LU  Xiao-Yu, WANG  Jin-Hua, CHEN  Yue, HUANG  Mei, ZHOU  Qi, XU  Chao-Yi   

  1. 1.Food Safety Inspection Center,Beijing Entry-Exit Inspection and Quarantine Bureau;2.College of Science,Beijing University of Chemical Technology
  • Online:2008-05-15 Published:2011-08-26

摘要: 应用超高效液相色谱-串联质谱仪(UPLC-MS-MS)研究了草莓中杀虫双残留的测定方法。样品用甲醇-水(1:1,V/V)提取,无需经过任何净化过程;以等梯度流动相、经ACQUITY UPLC HSS T3超高效液相色谱柱分离;以电喷雾负离子(ESI-)和多反应监测模式(MRM)进行MS测定。结果表明:杀虫双添加水平为0.05、0.10和0.20mg/kg时,回收率为65.3%~91.5%;相对标准偏差为7.8%~11.8%;方法检出限为3.0μg/kg。本方法仅需约1min的检测时间,且具有很高的灵敏度和准确度,能够满足草莓及其制品中残留杀虫双的快速、高灵敏检测分析。

关键词: 杀虫双残留, 草莓及其制品, UPLC-MS-MS

Abstract: A ultra performance liquid chromatography with electrospray tandem mass spectrometry(UPLC-MS-MS) method was developed for the quantitative determination of bisultap residue in raw strawberry and its processed products.The samples were extracted by methanol-water(1:1,V/V) without any purifying treatments,were separated by ACQUITY UPLC HSS T3 UPLC column with equal gradient mobile phase,and detected by electrospray tandem mass spectrometry with ES-and multiple reaction monitoring(MRM) modes.Results showed that the mean recoveries range from 65.3% to 91.5% with relative standard deviations in the range of 7.8%~11.8% when the spiked concentrations of bisultap are 0.05,0.10 and 0.20 mg/kg respectively,and the limit of detection is 3.0 μg/kg.The detection operation can be completed within 1 minute with high sensitivity and accuracy,and is suitable for the fast qualitative and quantitative detection for bisultap residue in strawberry samples.

Key words: bisultap residues, strawberry and strawberry products, UPLC-MS-MS