酶联免疫法与LC-MS/MS法测定水产品中呋喃唑酮代谢物

食品科学 ›› 2008, Vol. 29 ›› Issue (5): 363-366.

酶联免疫法与LC-MS/MS法测定水产品中呋喃唑酮代谢物

沈美芳, 宋红波, 丁涛, 吴光红, 沈崇钰, 吴蓓琦

江苏省水产质量检测中心农业部渔业产品质量监督检验测试中心；江苏省水产质量检测中心；农业部渔业产品质量监督检验测试中心；江苏出入境检验检疫局动植物与食品检测中心；国家蜂产品基准实验室；

出版日期:2008-05-15 发布日期:2011-08-26

Enzyme Linked Immunosorbent Assay(ELISA) and LC-MS/MS for Determination of Furazolidone Residues in Aquatic Products

SHEN Mei-Fang, SONG Hong-Bo, DING Tao, WU Guang-Hong, SHEN Chong-Yu, WU Bei-Qi

1.Jiangsu Provincial Aquatic Products Analysis and Testing Center,Fishery Products Quality Supervision and Testing Center(Nanjing),Ministry of Agriculture;2.Jiangsu Entry-Exit Inspection and Quarantine Bureau,National Bee-product Reference Laboratory

Online:2008-05-15 Published:2011-08-26

摘要/Abstract

摘要： 运用酶联免疫法和LC-MS/MS法对水产品中呋喃唑酮代谢物残留进行测定。样品经2-硝基苯甲醛衍生化并用乙酸乙酯提取。向样品中分别添加0.60、2.00、6.00μg/kg三个浓度水平的呋喃唑酮代谢物时,酶联免疫法平均回收率分别为99.2%、96.0%和100.0%,变异系数(RSD)为1.8%～12.6%,最低检测限为0.3μg/kg。LC-MS/MS法平均回收率分别为90.8%、94.3%和86.3%,RSD为6.2%～14.3%,最低检测限为0.1μg/kg。用酶联免疫法对150个样品进行检测,筛选出3个阳性样品,经LC-MS/MS确证为阳性,未发现假阳性样品,且测定结果基本一致。研究表明酶联免疫法重复性较好、准确度较高,是一种水产品中呋喃唑酮代谢物残留的快速筛选方法;液-质/质联用法灵敏度高,适用于阳性样品的确证和精确定量。

关键词: 水产品, 呋喃唑酮代谢物, 酶联免疫法, 液相质谱-电喷雾质谱

Abstract: ELISA and LC-MS/MS for the analysis of furazolidone(AOZ) residues in aquatic products were studied.Samples were derivatived with 2-nitrobenzaldehyde and extracted with ethyl acetate.When the samples were spiked with 0.60,2.00 and 6.00μg/kg of AOZ,the average recoveries are 99.2%,96.0% and 100.0% respectively by ELISA,while the relative standard deviation(RSD) is 1.8%～12.6% with detection limit of 0.3 μg/kg.The average recoveries are 90.8%,94.3% and 86.3% respectively by LC-MS/MS and RSD is 6.2%～14.3% with detection limit of 0.1μg/kg.Three positive samples analyzed by ELISA were confirmed by LC-MS/MS and no false positive samples were found.The results are basically the same.The results showed that ELISA is a rapid and exact method with good reproducibility suitable for the screen of AOZ positive samples in aquatic products.Hence LC-MS/MS is sensitive and suitable for determination of positive samples.

Key words: aquatic products, furazolidone, ELISA, LC-MS/MS

沈美芳, 宋红波, 丁涛, 吴光红, 沈崇钰, 吴蓓琦. 酶联免疫法与LC-MS/MS法测定水产品中呋喃唑酮代谢物[J]. 食品科学, 2008, 29(5): 363-366.

SHEN Mei-Fang, SONG Hong-Bo, DING Tao, WU Guang-Hong, SHEN Chong-Yu, WU Bei-Qi. Enzyme Linked Immunosorbent Assay(ELISA) and LC-MS/MS for Determination of Furazolidone Residues in Aquatic Products[J]. FOOD SCIENCE, 2008, 29(5): 363-366.

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