食品科学 ›› 2008, Vol. 29 ›› Issue (6): 64-69.

• 基础研究 • 上一篇    下一篇

天然抗氧化物体外活性评价方法的优选与优化

 赵艳红, 李建科, 李国秀   

  1. 陕西师范大学食品工程系; 陕西师范大学食品工程系
  • 出版日期:2008-06-15 发布日期:2011-08-26

Optimization of Evaluation Methods for Activities of Natural Antioxidants in vitro

 ZHAO  Yan-Hong, LI  Jian-Ke, LI  Guo-Xiu   

  1. Department of Food Engineering, Shaanxi Normal University
  • Online:2008-06-15 Published:2011-08-26

摘要: 选用抗坏血酸(VC)和茶多酚标准品为实验对象,对目前常用的抗氧化体外活性检测方法进行了评价,通过稳定性、可靠性、精密度及重现性实验,最终确定了可行性大、稳定性好、精密度高、重现性好的天然抗氧化物体外活性评价方法体系,并对有些检测体系进行了优化。同时,用本实验筛选优化的方法对石榴多酚的抗氧化活性进行了实际样品测定。

关键词: 抗氧化活性, 自由基, 分光光度法

Abstract: With standard ascorbic acid (vitamin C) and tea polyphenols as test objects, some methods for detecting commonly used natural antioxidant activity in vitro were evaluated in this study. An evaluation method system for natural antioxidant activity in vitro was optimized with practicable feasibility, good stability, high precision and excellent reproducibility. Then the antioxidant activities of pomegranate polyphenols were detected by this method system, and satisfying results are obtained.

Key words: antioxidant activity, free radical, spectrophotometry