食品科学 ›› 2008, Vol. 29 ›› Issue (6): 123-126.

• 基础研究 • 上一篇    下一篇

几种柑橘果实白皮层中过氧化物酶特性的比较研究

 蒲彪, 李庆   

  1. 四川农业大学食品科学系; 四川农业大学食品科学系
  • 出版日期:2008-06-15 发布日期:2011-08-26

Study on Comparison of Characteristics of Peroxidase in Albedo of Citrus Fruit

 PU  Biao, LI  Qing   

  1. Department of Food Science, Sichuan Agricultural University
  • Online:2008-06-15 Published:2011-08-26

摘要: 分离出柑橘果实的白皮层,充分切细使其粒径为0.5~1mm,用冷磷酸缓冲液浸提24h后四层纱布过滤,所得过氧化物酶(POD)粗酶液用于酶特性研究。结果表明,几种柑橘果实之间酶活性差异很大,最适宜的pH和温度均为5.0和40~60℃;随着底物浓度的升高,酶活力也升高,并在H2O2为0.07%时达到饱和状态。以塔希提柠檬白皮层中POD为研究对象,添加不同剂量的VC和Na2SO3作抑制剂,在浓度分别达到0.1%和0.5%时POD活力受到明显长时抑制。

关键词: 柑橘白皮层, 过氧化物酶(POD), 特性

Abstract: Albedo was chopped up to the diameter of 0.5~1 mm, which was separated from citrus fruits. The thin albedo was soaked in 0.2 mol/L sodium phosphate buffer at pH 6.0 for about 24 hours. Then, the resultant suspension for studying the POD characteristics was filtered through a four-fold layer of muslin. The results showed that POD activities among severl species of citrus are different relatively, and the optimum pH and temperature for the POD are 5.0 and 40~60 ℃, respectively. The POD activity increases with the increase of substrate concentration (H2O2) concentration, and reaches saturaed point at 0.07% H2O2. Moreover, both 0.1% VC and 0.5% Na2SO3 are strong inhibitors for POD activity in Tahiti albedo.

Key words: albedo of citrus fruit, POD, characteristics