食品科学 ›› 2008, Vol. 29 ›› Issue (6): 234-236.

• 生物工程 • 上一篇    下一篇

牛奶中植物成分的PCR检测方法研究

 覃芳芳, 邓鸿铃, 郭新东, 罗海英, 吴玉銮   

  1. 广州市产品质量监督检验所 国家加工食品质量监督检验中心; 广州市产品质量监督检验所; 国家加工食品质量监督检验中心
  • 出版日期:2008-06-15 发布日期:2011-08-26

Detection of Plant Ingredient in Milk by PCR Method

 QIN  Fang-Fang, DENG  Hong-Ling, GUO  Xin-Dong, LUO  Hai-Ying, WU  Yu-Luan   

  1. Guangzhou Product Quality Supervision and Testing Institute, National Centre for Quality Supervision and Testing of Processed Food (Guangzhou)
  • Online:2008-06-15 Published:2011-08-26

摘要: 建立了应用PCR技术鉴别牛奶饮料中植物成分的方法。将酚仿抽提DNA的方法与前处理相结合,得到了一种快速简便的从牛奶中提取DNA的方法,整个DNA提取的过程时间为2h左右。分析发现提取的牛奶饮料DNA完全可以进行PCR检测,并且该方法可检测到牛奶中掺入的植物成分低至0.1%。

关键词: DNA, PCR, 牛奶, 植物成分, 掺入

Abstract: The PCR technology was used in the detection of plant ingredient in milk drink. Combined with pre-operation and phenol-chloroform extraction, a rapid and simple method of DNA extraction from milk drinks was found, and DNA extraction took only two hours. The quality of DNA is sufficient for PCR analysis. Moreover, 0.1% plant ingredient in milk drink can be detected by this method.

Key words: DNA, PCR, milk, plant element, adulteration