食品科学 ›› 2008, Vol. 29 ›› Issue (3): 336-339.

• 生物工程 • 上一篇    下一篇

自溶法从啤酒废酵母中制取多肽的研究

 凌秀梅, 邱树毅, 黄永光, 佘勇   

  1. 贵州大学发酵工程与生物制药贵州省重点实验室; 成都市双流质量技术检测所 贵州贵阳550003 成都市双流质量技术检测所; 四川成都610200; 贵州贵阳550003;
  • 出版日期:2008-03-15 发布日期:2011-08-24

Autolyzing Preparation of Peptides from Waste Yeast

 LING  Xiu-Mei, QIU  Shu-Yi, HUANG  Yong-Guang, SHE  Yong   

  1. 1.Guizhou Provincial Key Laboratory of Fermentation Engineering and Biopharmacy, Guizhou University, Guiyang 550003, China; 2.Shuangliu Institute of Calibration and Testing for Quality and Technical Supervision, Chengdu 610200, China
  • Online:2008-03-15 Published:2011-08-24

摘要: 对啤酒废酵母制取多肽的过程进行了研究,采用正交法确定了自溶的最佳条件,并用SephadexG-25凝胶层析分离出10种多肽,其含量随着自溶的时间不同而有所变化,通过氨基酸自动分析仪对多肽氨基酸和游离氨基酸的种类和含量进行测定,结果发现自溶60h后多肽总含量达到744mg/100ml。

关键词: 自溶, 多肽, 凝胶层析

Abstract: Both the preparation of yeast peptides and the optimum conditions of autolyzing were studied. 10 kinds of peptides were separated by SephadexG-25. The quantities of peptides changed according to the autolyzing time. The composion and content of amino acid was determined by amino acid analyzer. The results showed that the content of total peptides reaches 744 mg/100 ml.

Key words: autolyze peptide, gel filtration, chromatography