食品科学 ›› 2008, Vol. 29 ›› Issue (6): 350-352.

• 分析检测 • 上一篇    下一篇

RP-HPLC法测定不同芦荟中褪黑激素含量

 王英娟, 步怀宇, 贾敬芬, 索志荣   

  1. 西北大学生命科学学院; 西北大学分析科学研究所 陕西西安710069; 陕西西安710069;
  • 出版日期:2008-06-15 发布日期:2011-08-26

RP-HPLC Analysis of Melatonin Content in Different Aloe Tissues

 WANG  Ying-Juan, BU  Huai-Yu, JIA  Jing-Fen, SUO  Zhi-Rong   

  1. 1.College of Life Science, Northwest University, Xi’an 710069, China; 2.Institute of Analytical Science, Northwest University, Xi’an 710069, China
  • Online:2008-06-15 Published:2011-08-26

摘要: 目的:建立测定芦荟组织中有效成分褪黑素的反相高效液相色谱分析方法,并用该方法测定不同品种的芦荟组织。方法:以SymmetryC18(150mm×4.6mm,5.0μm)为色谱柱;甲醇:水(1:1)为流动相,流速为1.0ml/min;检测波长为223nm,灵敏度为2.00AUFS。结果:褪黑素浓度在2.0~200.0pg/ml(r=0.9892)范围内线性关系良好,平均加样回收率为98.8%(n=6),RSD=1.13%。结果表明,利用RP-HPLC法分析不同芦荟中褪黑激素含量简便、易行。芦荟组织中褪黑素含量随品种、组织不同而不同,其中花高于同种的叶。库拉索芦荟中的褪黑素含量远高于其它不同品种的芦荟的同类组织。

关键词: RP-HPLC, 芦荟, 褪黑素

Abstract: Objective: To establish a sensitive RP-HPLC method for determining the content of the active ingredient-melatonin in the different species of aloes and their tissues. Method: To make Making use of Symmetry C18 150 mm×4.6 mm, 5.0 μm) as column, with the flow of carbinol: water in the ratio 1:1, at the flow rate 1.0 ml/min. The inspected wavelength is 223 nm with sensitivity degree 2.00 AUFS. Result: The linear range for melatonin is perfect within 2.0~200.0 pg/ml (r=0.9892) with the average recovery(n = 6) 98.8% and RSD 1.13%. The method is simple and easy for determinfim of the melatonin content in aloe. The melatonin content varies in different species of aloes and their different tissues, and the content in the flower is higher than that in the leaf of aloe. The content of melatonin in Aloe vera L. is the highest.

Key words: RP-HPLC, aloe, melatonin