食品科学 ›› 2008, Vol. 29 ›› Issue (6): 364-367.

• 分析检测 • 上一篇    下一篇

RP-HPLC法测定鸡胚蛋及鸡蛋中性激素的残留量

 高伟, 张培正, 宫玮   

  1. 云南农业大学食品科技学院; 山东农业大学食品科学与工程学院 云南昆明650201; 云南昆明650201; 山东泰安271018;
  • 出版日期:2008-06-15 发布日期:2011-08-26

Determination of Residual Free Sex Hormons in HY-LINE Variety Brown Embryo and Egg by RP-HPLC

 GAO  Wei, ZHANG  Pei-Zheng, GONG  Wei   

  1. 1.Academy of Food Science and Technology,Yunnan Agricultural University, Kunming 650201, China; 2.College of Food Science and Engineeing, Shandong Agricultural University, Taian 271018, China
  • Online:2008-06-15 Published:2011-08-26

摘要: 目的:建立了RP-HPLC法测定鸡胚蛋及鸡蛋中己烯雌酚、丙酸睾酮激素残留。方法:采用WatersXTerraC18柱(150mm×4.6mm,5μm),流动相为甲醇:乙腈:水(2:2:1,V/V/V),流速为1ml/min,柱温为40℃,检测波长为240nm。用外标法定量,测定激素残留量。结果与结论:该方法线性范围为0.05~3.500μg/ml,标准曲线线性关系良好,其回归方程和相关系数分别为:己烯雌酚y=21734x+963.18,R2=0.9991;丙酸睾酮y=28967x-475.62,R2=0.9995。最低检测限为己烯雌酚0.25ng,丙酸睾酮0.33ng。回收率在78.7%~98.4%之间。相对标准偏差小于2.1%。

关键词: 己烯雌酚, 丙酸睾酮, RP-HPLC, 鸡胚蛋

Abstract: Objective: To determine the content of diethylstilbestrol and testosterone in HY-LINE Variety Brown chick embryo and egg. Methods: RP-HPLC method was used to determine the content of diethylstilbestrol and testosterone in chick embryo. The analysis was carried out on Waters XTerra C18 column (150 mm×4.6 mm, 5μm) with the mobile phase methanol-acetonitrile-water(2:2:1, V/V/V), flow rate 1.0 ml/min, temperature 40 ℃and wavelength 240 nm. Results and conclusion: The method is simple and has a good linear relationship in the range of 0.05~3.500 μg/ml. The linear equation of diethylstilbestrol is y = 21734x + 963.18 with correlation coefficient 0.9991, and the linear equation of testosterone is y = 28967x-475.62, with correlation coefficient 0.9995. The limit of detection of diethylstilbestrol is 0.25 ng, and that of testosterone is 0.33 ng. The average recovery ranges from 78.7% to 98.4%. The relative standard deviation is less than 2.1%.

Key words: diethylstilbestrol, testosterone proprionate, RP-HPLC, chick embryo