海参对糖尿病大鼠肾脏的保护作用及其机制的研究

食品科学 ›› 2012, Vol. 33 ›› Issue (1): 252-256.

海参对糖尿病大鼠肾脏的保护作用及其机制的研究

龙腾腾，王静凤，赵芹，张珣，李晓林，薛长湖，李兆杰*

中国海洋大学食品科学与工程学院

收稿日期:2011-03-14 修回日期:2012-01-06 出版日期:2012-01-15 发布日期:2012-01-12
通讯作者: 薛长湖 E-mail:xuech@ouc.edu.cn
基金资助:
海洋公益性行业科研专项;国际科技合作项目

Protective Effect and Mechanism of Apostichopus japonicus on Kidney of Diabetic Rats

Received:2011-03-14 Revised:2012-01-06 Online:2012-01-15 Published:2012-01-12

摘要/Abstract

摘要： 目的：研究海参对链脲佐菌素(STZ)诱导的糖尿病大鼠肾脏的保护作用，并探讨其作用机制。方法：采用一次性腹腔注射STZ的方法建立糖尿病大鼠模型，在长期高血糖环境下造成对肾脏的损伤。灌胃海参(800mg/(kg·d)8周后，分别检测大鼠空腹血糖值、尿糖含量、肾脏指数和尿总蛋白、微量白蛋白(mAlb)、N-乙酰-β-D-氨基葡萄糖苷酶(NAG)排泄率；采用RT-PCR方法测定大鼠肾脏中过氧化物酶体增殖物激活受体γ(PPARγ)、转化生长因子β1(TGF-β1)和结缔组织生长因子(CTGF) mRNA的表达水平。结果：海参能显著降低STZ诱导的糖尿病大鼠空腹血糖值、尿糖含量、肾脏指数以及尿总蛋白、mAlb和NAG排泄率(P＜0.05或P＜0.01)，并且能显著上调糖尿病大鼠PPARγmRNA，下调TGF-β1和CTGF(P＜0.05) mRNA的表达。结论：海参对糖尿病大鼠具有良好的降血糖及改善肾脏滤过功能的作用，其机制可能与促进肾脏中PPARγmRNA的表达，抑制TGF-β1和CTGF mRNA的表达有关，最终实现对糖尿病大鼠肾脏的保护作用。

关键词: 糖尿病, 日本刺参, 过氧化物酶体增殖物激活受体γ, 转化生长因子β1, 结缔组织生长因子

Abstract: Objective: To explore the protective effect of Apostichopus japonicus on the kidney of streptozotocin (STZ)-induced diabetic rats and its possible mechanisms. Methods: A diabetic rat model was developed by a single intraperitoneal injection of STZ at the dose of 60 mg/kg and kidney damage was induced by long-term high levels of blood glucose. The diabetic rats were administered with Apostichopus japonicus at the daily dose of 800 mg/kg for 8 consecutive weeks. The changes in fasting blood glucose, urine glucose, renal index, and the excretion amounts of urine protein, mAlb and NAG were determined. The mRNA expression of peroxisome proliferation activated receptor-γ (PPARγ), transforming growth factor beta 1 (TGF-β1) and connective tissue growth factor (CTGF) in kidney were assessed by RT-PCR. Results: Apostichopus japonicus showed remarkable a suppressive effect on fasting blood glucose, uric glucose, kidney index and the excretion amounts of protein, mAlb and NAG in diabetic rats. The expression level of PPARγ mRNA was markedly increased, while the mRNA expression levels of TGF-β1 and CTGF were effectively suppressed by Apostichopus japonicus. Conclusion: Apostichopus japonicus can remarkably reduce blood glucose through improving the expression of PPARγ and restraining the expression of TGF-β1 and CTGF, which results in a protective effect on kidney damage.

Key words: diabetes, Apostichopus japonicus, PPARγ, TGF-β1, CTGF

中图分类号:

龙腾腾，王静凤，赵芹，张珣，李晓林，薛长湖，李兆杰*. 海参对糖尿病大鼠肾脏的保护作用及其机制的研究[J]. 食品科学, 2012, 33(1): 252-256.

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