食品科学 ›› 2007, Vol. 28 ›› Issue (7): 60-62.

• 基础研究 • 上一篇    下一篇

蒜氨酸酶的光谱特征研究

 李瑜, 许时婴, 庞凌云, 乔明武   

  1. 河南农业大学食品科学技术学院; 江南大学食品学院 教育部食品科学与安全重点实验室; 河南郑州450002江南大学食品学院; 教育部食品科学与安全重点实验室; 江苏 无锡 214036; 江苏无锡214036; 河南郑州450002;
  • 出版日期:2007-07-15 发布日期:2011-10-24

Study on Spectral Characteristics of Alliinase

 LI  Yu, XU  Shi-Ying, PANG  Ling-Yun, QIAO  Ming-Wu   

  1. 1.College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China; 2.School of Food Science and Technology, Southern Yangtze University, Key Laboratory of Food Science and Safety, Ministry of Education, Wuxi 214036, China
  • Online:2007-07-15 Published:2011-10-24

摘要: 本实验采用Sephacryl S-200 凝胶柱对新鲜大蒜中的蒜氨酸酶进行了分离纯化,并对纯化后的蒜氨酸酶的紫外 - 可见光谱、荧光发射光谱和圆二色性等光谱学特性进行了研究。结果表明,蒜氨酸酶的α- 螺旋结构大约占27%~30%,几乎不存在β- 折叠结构,β- 转角结构大约占 30% 左右,无规卷曲所占比例大约占 40% 左右。

关键词: 大蒜, 蒜氨酸酶, 光谱学特性

Abstract: In this paper, alliinase from fresh garlic was purified with Sephacryl S-200 gel column and the spectral characteristic of ultraviolet/visible spectra, fluorescence and CD spectroscopy were studied. The results showed that in alliinase α-helix structure accounts for about 27%~30%, β-pleated sheet structure almost doesn’t exist, β-turn structure accounts for about 30%, and random coil structure accounts for about 40%.

Key words: garlic, alliinase, spectral characteristics