聚乙二醇修饰对牛乳β-乳球蛋白抗原性的影响

食品科学 ›› 0, Vol. ›› Issue (): 234-237.

聚乙二醇修饰对牛乳β-乳球蛋白抗原性的影响

蔡小飞，钟俊桢，刘成梅*，刘伟，徐雨佳，罗舜菁

南昌大学食品科学与技术国家重点实验室

收稿日期:2011-06-20 修回日期:2011-08-09 出版日期:2011-09-15 发布日期:2011-08-30
通讯作者: 刘成梅 E-mail:chengmeiliu@yahoo.com.cn
基金资助:
DHPM胁迫下β-乳球蛋白构象变化与致敏性间的关系

Effect of PEG Modification on Antigenicity of β-Lactoglobulin

Received:2011-06-20 Revised:2011-08-09 Online:2011-09-15 Published:2011-08-30

摘要/Abstract

摘要： 通过不同修饰反应物质的量比、修饰反应时间、pH值，用单甲氧基聚乙二醇琥珀酰亚胺酯(SC-mPEG)对牛乳β-乳球蛋白(β-LG)进行修饰，研究其抗原性的变化。利用间接竞争ELISA检测结合产物的抗原性，结果显示不同条件下PEG修饰后的β-乳球蛋白的抗原性最高降低率，反应8h时为70.2%。三硝基苯磺酸(TNBS)法测定不同条件反应后样品修饰度，反应8h时最高修饰度为39.1%。结果表明修饰反应时间为SC-mPEG修饰β-LG后其抗原性降低的主要因素。

关键词: β-乳球蛋白, 聚乙二醇, ELISA, 抗原性

Abstract: β-Lactoglobulin (β-LG) was modified by suecinimidyl carbonate methoxy polyethlene glycol (SC-mPEG) under different conditions of molar ratio, reaction time and pH. The change of its antigenicity was also studied. Indirect competitive ELISA was used to detect the antigenicity of the SC-mPEG-β-LG mixture. The results indicated that the highest reduction rate of antigenicity for modified products under different conditions was 70.2% at the reaction time of 8 h and at the same time, the highest modification degree detected by TNBS was 39.1%. Meanwhile, reaction time was the major factor affecting antigenicity reduction of β-LG modified by SC-mPEG.

Key words: β-lactoglobulin, polyethylene glycol, ELISA, antigenicity

中图分类号:

TS252.1

蔡小飞，钟俊桢，刘成梅*，刘伟，徐雨佳，罗舜菁. 聚乙二醇修饰对牛乳β-乳球蛋白抗原性的影响[J]. 食品科学, 0, (): 234-237.

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