食品科学 ›› 2006, Vol. 27 ›› Issue (3): 75-78.

• 基础研究 • 上一篇    下一篇

紫球藻的培养及其硫酸多糖的分离纯化

  夏海锋, 姚善泾   

  1. 浙江大学化学工程与生物工程学系
  • 出版日期:2006-03-15 发布日期:2011-09-06

Cultivation of Porphyridium spp. and Separation of Its Sulfated Polysaccharide

   Xia-Hai-Feng, YAO  Shan-Jing   

  1. Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China
  • Online:2006-03-15 Published:2011-09-06

摘要: 利用优化了的海水培养基和简易光生物反应器对紫球藻进行培养,建立了一套从培养液中直接分离纯化紫球藻多糖(PSP)的工艺,并对纯化后的PSP进行了初步分析。实验表明,本方法培养的紫球藻生物量达到了干重2.1g/L,分离后粗PSP产量为0.378g/L,离子交换得率约为78%。经气相色谱分析PSP主要由木糖、葡萄糖和半乳糖组成,质量比约为13.68:7.83:5.23。红外光谱显示该多糖具有一般多糖的特征吸收峰,其中六碳糖为吡喃型。PSP为硫酸酯化多糖,硫酸基团含量约为4%。

关键词: 紫球藻, 紫球藻多糖, 培养, 分离纯化, 气相色谱, 红外光谱

Abstract:  Culturing Porphyridium spp. with optimized culture medium and simple photobioreactor was investigated. Apractical technical process on direct separation of Polysaccharide of Porphyridium(PSP) from broth was established, and PSPwas primarily analyzed. The results showed that the biomass of cultured Porphyridium reached 2.1g dry weight per litre, yieldof primary PSP 0.378g/L and efficiency of ion exchange about 78%. PSP was basically composed of xylose, glucose and galactose,with a mass ratio 13.68:7.83:5.23 respectively. The result by IR analysis indicates that PSP has its characteristic absorption peak,and its hexose is of pyranoid form while PSP is sulfated polysaccharide, and its content of –SO3- is about 4%.

Key words: Porphyridium spp. PSP, cultivation, separation of sulfated polysaccharides