食品科学 ›› 2006, Vol. 27 ›› Issue (3): 82-86.

• 基础研究 • 上一篇    下一篇

水溶性苦瓜多糖的分离纯化及生物学活性的研究

 吴笳笛, 陈红漫   

  1. 沈阳农业大学生物技术学院; 锦州医学院畜牧兽医学院
  • 出版日期:2006-03-15 发布日期:2011-09-06

Study on Seperation, Purification and Biological Activity for Soluble Polysaccharide from Momordica charantia

 WU  Jia-Di, CHEN  Hong-Man   

  1. 1.College of Biological Technology, Shenyang Agricultural University, Shenyang 110161, China;2.College of Animal Husbandry and Veterinary, Jinzhou Medical College, Jinzhou 121000, China.
  • Online:2006-03-15 Published:2011-09-06

摘要: 本文建立了苦瓜水溶性多糖的提取纯化方法,并对其理化性质及生物学活性进行检测。采用双酶法得到的苦瓜水溶性多糖,通过正交试验确定其最佳水解条件为:纤维素酶-提取温度50℃,pH值6.0,酶用量5%;中性蛋白酶-水解时间48h,pH值7.0,酶用量10%。多糖粗品经DEAE-32、SephadexG-75柱层析得到单一组分,命名为PMCⅠ。PMCⅠ的酸性水解液经薄层层析分析,其单糖组分别为葡萄糖、半乳糖、甘露糖和鼠李糖。对PMCⅠ生物功能的研究表明,其对羟基自由基、超氧阴离子自由基均有清除作用,IC50分别为0.0017mg/ml和0.005mg/ml,最大抑制率可达100%,证实其具有抗氧化的活性。抑菌实验表明其对金黄色葡萄球菌、大肠杆菌、鼠伤寒沙门氏菌、枯草杆菌等病原菌均有抑制作用,IC50分别为0.10、0.15、0.13、0.13mg/ml,其中以金黄色葡萄球菌最为明显。

关键词: 苦瓜多糖, 分离纯化, 纤维素酶, 生物学活性

Abstract: The method of extracting and purifying polysaccharide from Momordica charantia was suggested in this paper. Itsphysical and chemical characteristics and biological activity were determined. The polysaccharide was extracted from Momordicacharantia by adding two species of enzymes. The optimum hydrolysis conditions were got. The polysaccharide was furtherpurified with DEAE-32 column chromatography and Sephadex G-75 column chromatography respectively until the single peakwas got, and named as PMCⅠ. The acidic hydrolysis solution of PMCⅠwas analysed by means of TLC: its constituentsrespectively glucose, galactose, mannose, rhamnose. The study for its biological activity showed that it could eliminate free radicalssuch as ·OH and O2·, and its IC50 for ·OH and O2· were respectively 0.0017mg/ml and 0.005mg/ml. So it showed theactivity of antioxidant. The experiments of inhibiting germs show that PMCⅠcan inhibit germs such as S.Aureus, E.coli, B.subtilusand S.typhimurium. The IC50 for those were respectively 0.10, 0.15, 0.13, and 0.13mg/ml, the most remarkable of which was S.Aureus.

Key words: Momordica charantia polysaccharide, seperation purification, cellulase, activity- -