食品科学 ›› 2006, Vol. 27 ›› Issue (3): 96-99.

• 基础研究 • 上一篇    下一篇

原生质体融合提高嗜酸乳杆菌耐酸及耐胆盐能力

 王玉华, 张桂荣, 刘景圣   

  1. 吉林农业大学食品工程学院; 长春教育学院;
  • 出版日期:2006-03-15 发布日期:2011-09-06

Improvement Study on Acid and Bile Tolerance of Lactobacillus acidophilus Protoplast Fusion

 WANG  Yu-Hua, ZHANG  Gui-Rong, LIU  Jing-Sheng   

  1. 1.Department of Food Engineering, Jilin Agricultural University, Changchun 130118, China;2.Changchun Institute of Education, Changchun 130046, China
  • Online:2006-03-15 Published:2011-09-06

摘要: 两株不同来源嗜酸乳杆菌La-w1和La-w2进行原生质体融合,获得一株耐酸和耐胆盐能力强的菌株La-F1,并对其生长特性进行研究。结果表明,融合菌株La-F1耐酸性和耐胆盐能力明显高于初始菌株,其生长特性适于乳酸发酵食品要求。

关键词: 嗜酸乳杆菌, 原生质体融合, 耐酸及耐胆盐

Abstract: Lactobacillus acidophilus La-w1 and La-w2 from different sources were successfully fused by using the protoplastfusion technique. Fusion hybrid La-F1 was obtained, and its growing characteristics cultivated in skim milk for 48 hours at 37℃ werestudied. Results showed that La-F1 has better acid and bile tolerance than initial strains and the fermented characterics meet therequirement of lactic acid fermented food.

Key words: Lactobacillus acidophilus, protoplast fusion, acid and bile tolerance