食品科学 ›› 2007, Vol. 28 ›› Issue (8): 307-309.

• 生物工程 • 上一篇    下一篇

杜仲愈伤组织继代培养及抑制褐化的研究

 邱晓芳, 朱笃, 张志斌, 涂艺声, 游海, 王曼莹   

  1. 江西师范大学生命科学学院江西省亚热带植物资源保护与利用重点实验室; 江西师范大学生命科学学院江西省亚热带植物资源保护与利用重点实验室 江西南昌330022; 江西南昌330022;
  • 出版日期:2007-08-15 发布日期:2011-10-24

Study on Callus Subculture and Darkening Inhibition of Eucommia ulmoides

 QIU  Xiao-Fang, ZHU  Du, ZHANG  Zhi-Bin, TU  Yi-Sheng, YOU  Hai, WANG  Man-Ying   

  1. Jiangxi Provincial Key Laboratory of Protection and Utilization of Subtopic Plant Resources, College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China
  • Online:2007-08-15 Published:2011-10-24

摘要: 对杜仲愈伤组织的继代培养及其褐化现象的抑制进行了研究,结果表明:B5培养基+0.5mg/LNAA+1.0mg/L6-BA是杜仲愈伤组织继代培养和褐化控制的适合培养基;培养基中添加0.5%活性炭(AC)能有效的抑制杜仲愈伤组织的褐化;与中强光和红光相比,蓝光和黑暗能减轻组织的褐化。

关键词: 杜仲, 愈伤组织, 继代培养, 褐化

Abstract: In this paper, the callus subculture and its darkening control of Eucommia ulmoides were investigated. The results indicated that B5+0.5 mg/L NAA +1.0 mg/L 6-BA is the optimum medium for callus subculture and darkening control. It was the most efficient anti-darkening treatment that the 0.5% activated charcoal (AC) was added in base medium. Compared with high intensity light and red light, the callus growing under blue light or dark showed the lighter darkening.

Key words:  , Eucommia ulmoides; callus; subculture; darkening;