食品科学 ›› 2007, Vol. 28 ›› Issue (8): 337-340.

• 分析检测 • 上一篇    下一篇

胶体金免疫层析法半定量检测鸡肉中磺胺嘧啶残留

 王喜亮, 易俊东, 金秀娥, 李奎, 卢芳, 熊宁, 刘群, 刘百红, 毕丁仁   

  1. 华中农业大学动物医学院; 湖北省兽药监察所; 华中农业大学动物医学院 湖北武汉430070; 湖北武汉430064; 湖北武汉430070;
  • 出版日期:2007-08-15 发布日期:2011-10-24

Semi-quantitative Detection of Sulfadiazine by Immunochromatographic Assay in Chicken Meats

 WANG  Xi-Liang, YI  Jun-Dong, JIN  Xiu-E, LI  Kui, LU  Fang, XIONG  Ning, LIU  Qun, LIU  Bai-Hong, BI  Ding-Ren   

  1. 1.College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; 2. Hubei Provincial Institute of Veterinary Drug Control, Wuhan 430064, China
  • Online:2007-08-15 Published:2011-10-24

摘要: 本研究采用柠檬酸三钠还原法制备胶体金,用胶体金标记磺胺嘧啶单克隆抗体,磺胺嘧啶竞争物包被于硝酸纤维素膜,制成免疫层析快速检测试纸条。该试纸条可以在15min内完成对磺胺嘧啶残留的半定量检测,灵敏度为5ng/ml,与其它磺胺类药物无交叉反应,在鸡肉中的回收率为71%~95%;用试纸条与高效液相法对来自动物试验的56份鸡肉样品进行对比检测。以我国磺胺嘧啶残留限量标准(100ng/g)为标准,二者符合率为100%。该方法灵敏度高,简便快速,无需特殊仪器设备,有良好的开发应用前景。

关键词: 胶体金, 免疫层析, 鸡肉, 磺胺嘧啶

Abstract: Colloidal gold was prepared with reducing reaction of sodium citrate and was coulped with monocolonal anti-Sulfadiazine in this article. BSA-sulfadiazine was coupled with the nitrocellulose membranes to prepare immunochromatographic strips. With this method, semi-quantitative detection of sulfadiazine can be finished in less than 15 min. The sensitivity to sulfadiazine is 5 ng/ml, and no cross-reactivity with other sulfonamides. Good recovery rates are determined from 71% to 95% in chickens meats. Based on maximum residue level of sulfadiazine (100 ng/g) in China, this method was compared with with high-performance liquid chromatographic (HPLC) by assaying 56 chicken meat samples taken from animal experiment. The agreement rates are 100%. This method is a sensitive, simple, rapid assay without the need of special apparatus, so it is of good practical value.

Key words: colloidal gold, immunochromatography, chicken meats, sulfadiazine