食品科学 ›› 2006, Vol. 27 ›› Issue (3): 137-141.

• 工艺技术 • 上一篇    下一篇

金属离子对红发夫酵母的生长、细胞形态及虾青素合成的影响

华艳艳, 孙玉梅, 曹芳, 杨宇, 陈芳良   

  1. 大连轻工业学院生物与食品工程学院
  • 出版日期:2006-03-15 发布日期:2011-09-06

Effects of Metal Ions on Cell Growth, Cell Morphology and Astaxanthin Biosynthesis of Phaffia rhodozyma

HUA  Yan-Yan, SUN  Yu-Mei, CAO  Fang, YANG  Yu, CHEN  Fang-Liang   

  1. College of Biology and Food Engineering, Dalian Institute of Light Industry, Dalian 116034, China
  • Online:2006-03-15 Published:2011-09-06

摘要: 主要研究了Mn2+、Mg2+和Ca2+三种金属离子对红发夫酵母(Phaffiarhodozyma)的生长、细胞形态及虾青素生物合成的影响。结果表明,在种子培养基中添加Mn2+能明显提高红发夫酵母的β-D-葡萄糖苷酶的活性并改变细胞的形态;在发酵培养基中添加0.1g/LMn2+,能明显促进细胞生长及虾青素合成;用正交试验法优化得到发酵培养基中的金属离子浓度为:0.1g/LMn2+、0.7g/LMg2+、0.2g/LCa2+,在优化后的培养基中,细胞生长速度快,摇瓶发酵57h的虾青素产量达2.95mg/L,是不加金属离子情况下发酵产虾青素最大量(发酵72h)的1.31倍。

关键词: 红发夫酵母, 金属离子, 虾青素, 细胞形态, 发酵

Abstract:  The effects of different metal ions on cell growth, cell morphology and astaxanthin biosynthesis of Phaffia rhodozymawere studied. It indicated that Mn2+ showed clear positive effects on the β-D-glucosidase activity and cell morphology in seedculture medium. The initial ferment medium containing 0.1g/L Mn2+ could improve obviously the cell growth and astaxanthinbiosynthesis. The optimal conditions obtained by orthogonal experiment were: 0.1g/L Mn2+, 0.7g/L Mg2+ and 0.2g/L Ca2+ in theferment medium. Under the optimal conditions, the cell grows rapidly, and the astaxanthin yield reaches 2.95mg/L at 57 h, whichis 1.31 times of that under no added metal ions (at 72 h).

Key words: Phaffia rhodozyma, metal ions, astaxanthin, cell morphology, fermentation