食品科学 ›› 2006, Vol. 27 ›› Issue (4): 96-99.

• 基础研究 • 上一篇    下一篇

不同处理方法对分离7S、11S中抗原含量的影响

 胡晓苹, 薛文通, 刘晓毅, 燕平梅   

  1. 中国农业大学食品科学与营养工程学院
  • 出版日期:2006-04-15 发布日期:2011-09-13

Contents of Soybean Allergens in Separated 7S and 11S by Different Processing Methods

 HU  Xiao-苹, XUE  Wen-Tong, LIU  Xiao-Yi, YAN  Ping-Mei   

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Online:2006-04-15 Published:2011-09-13

摘要: 7S和11S是大豆的主要球蛋白,两者占全蛋白的70%。GlymBd30K、GlymBd28K和β-伴大豆球蛋白(7S球蛋白)的α-亚基(MW68k)是大豆中的主要致敏蛋白。本文主要用三种缓冲液的不同处理方法分离7S、11S,使大豆的主要致敏蛋白尽量少的存在于分离后的11S组分中,而主要集中在分离后的7S组分中。豆腐的硬度主要依赖于11S球蛋白,因此这为大豆蛋白脱敏的同时仍能保有良好的凝胶形成能力提供新思路。

关键词: 大豆, 抗原, 分离, 凝胶形成

Abstract: 7S and 11S are the main globins in soybean, amounting to 70 percent of the total soybean protein. Gly m Bd 30K, Gly m Bd 28K andα-Subunit of β-Conglycinin(MW 68k)are the main allergens of soybean. In this paper, different processing methods of 3 different buffer solutions were used to separate 7S and 11S. The results showed that the main allergens existed as little as possible in the separated 11S, with the main allergens mainly existed in the separated 7S. Since the hardness of tofu gel mainly depends on the nature of 11S, it is a new finding to remove the soybean allergens, while at the same time, the gel-forming ability of soybean protein is maintained.

Key words:  , soybean; allergen; separate; gel-forming;