食品科学 ›› 2006, Vol. 27 ›› Issue (4): 106-109.

• 基础研究 • 上一篇    下一篇

乳杆菌L2抗突变活性物质组成分析及部分理化性质研究

 苏伟, 曹郁生   

  1. 南昌大学中德联合研究院食品科学教育部重点实验室; 江西科技师范学院生命科学学院
  • 出版日期:2006-04-15 发布日期:2011-09-13

Analysis on Components and Physical and Chemical Characteristics of Antimutagenic Substance of Lactobacillus L2

 SU  Wei, CAO  Yu-Sheng   

  1. 1.Jiangxi-Germany Joint Research Institute, Nanchang University, Key Laboratory of Food Science of Ministry of Education, Nanchang 330047, China;2.College of Life Science, Jiangxi Science and Technology Normal University, Nanchang 330013, China
  • Online:2006-04-15 Published:2011-09-13

摘要:  本文对分离纯化的乳杆菌L2的抗突变活性物质的化学组成进行了研究,用苯酚-硫酸法测糖含量,用BCAProteinassaykit测蛋白含量,用氨基酸分析仪测各氨基酸组成。结果表明,该糖蛋白糖含量为41.3μg/ml,蛋白含量约为872μg/ml,两者的含量比约为1:20,总氨基酸含量为35.91%,其中含量较高的氨基酸为苯丙氨酸(Phe),其次为精氨酸。然后对该糖蛋白部分理化特性进行了研究,该物质分子量约10kDa,作用温度范围为20~60℃,作用pH范围是5~9,10~50mmol/L的K+对糖蛋白活性基本无影响,对活性影响最大的是Fe3+。

关键词: 乳杆菌L2, 抗突变活性物质, 理化性质

Abstract: In this study, chemical components of antimutagenic substance of L2 were assayed. The total sugar content of the glycoprotein was 41.3μg/ml by phenol-vitriol, and the protein content were 872μg/ml. The ratio of sugar and protein was nearly 1:20. In this active glycoprotein, the total amino acid contents were 35.91%, while the major amino acids were phenylalanine and arginine. Then, the physical and chemical characteristics of active glycoprotein were analyzed. Its molecular mass was 10kDa, its reactive tempeture range 20~60℃, pH range was 5~9. 10~50mmol/L K+ show little effect on its activity, while Fe3+ has the largest effect on it.

Key words:  , Lactobacillus L2; antimutagenic activity component; physical and chemical characteristics;