食品科学 ›› 2007, Vol. 28 ›› Issue (9): 343-345.

• 生物工程 • 上一篇    下一篇

雄烯二酮转化菌的复合诱变及性能检测

 杨英, 姜绍通, 胡锦艳, 赵俊平   

  1. 合肥工业大学生物与食品学院; 合肥工业大学生物与食品学院 安徽合肥230009安徽建筑工业学院环境工程系; 安徽合肥230022; 安徽合肥230009;
  • 出版日期:2007-09-15 发布日期:2011-11-22

Mutation Breeding of Producing AD Strain by Complex Mutation and Natural Capacity Identification

 YANG  Ying, JIANG  Shao-Tong, HU  Jin-Yan, ZHAO  Jun-Ping   

  1. 1.College of Food and Biological,Hefei University of Technology,Hefei 230009,China;2.Department of Environment Engineering,Anhui Institute of Architecture,Hefei 230022,China
  • Online:2007-09-15 Published:2011-11-22

摘要: 生物转化法生产雄烯二酮还未实现大规模工业化生产的主要原因之一是雄烯二酮转化率低。以Mycobac-teriumsp.BD696#为出发菌株,分别采用UV照射、60Co照射、UV+60Co复合诱变处理筛选获得一株高转化率突变株SP-UC-8#。其产生AD的能力较出发菌株提高了39.2%,且不再产生结构类似物雄二烯二酮(ADD),且传至第五代产率仍较为稳定,说明UV+60Co复合诱变是遗传育种的一种有效方法。

关键词: 诱变育种, 紫外光, 雄烯二酮, 生物转化

Abstract: One of key factors,which hindered producing androst-4-ene-3,17-dione(AD)on industrial scale is that the yield of androst-4-ene-3,17-dione is low.The microorganism of Mycobacterium sp.BD696# was treated by complex mutagenesis of UV,60Co and UV+60Co ray irradiation.And AD transformation productivity was 39.2% higher than that of its parent strain and no androst-1,4-ene-3,17-dione was obtained.The fifth stain also had stable transformation productivity.The results showed that complex mutagenesis of UV+60Co ray irradiation is a good methed for mutation breeding.

Key words: mutation breeding, UV light, androst-4-ene-3,17-dione, bioconversion