食品科学 ›› 2007, Vol. 28 ›› Issue (9): 398-401.

• 生物工程 • 上一篇    下一篇

诱变选育ε-聚赖氨酸产生菌突变株

 张海涛, 李燕, 欧杰, 蔡冰莹   

  1. 上海水产大学食品学院; 上海水产大学食品学院 上海200090; 上海200090;
  • 出版日期:2007-09-15 发布日期:2011-11-22

Mutation Breeding of Strain for Producing ε-polylysine

 ZHANG  Hai-Tao, LI  Yan, 欧Jie , CAI  Bing-Ying   

  1. College of Food Science and Technology,Shanghai Fisheries University,Shanghai 200090,China
  • Online:2007-09-15 Published:2011-11-22

摘要: 以白色链霉菌(Streptomyces albulus)SF-21为出发菌株,对其进行微波诱变、硫酸二乙酯诱变和紫外诱变。在诱变过程中经瓶初筛、复筛获得了高产ε-聚赖氨酸的菌株,ε-聚赖氨酸的产量达0.848g/L,比出发菌株提高了41.3%。经5次传代发酵表明该菌株稳定。

关键词: 诱变, &epsilon, -聚赖氨酸, 白色链霉菌

Abstract: Streptomyces albulus was used as the starting strain and treated with microwave mutation,chemical revulsant and UV irradiation.During mutagenesis process,a high producing ε-polylysine strain was obtained which produces 0.848 g/L increasing 41.3% compared with the staring strain.The mutant showed stable genetic property.

Key words: mutation; &epsilon, -polylysine; Streptomyces albulus;